ArticleThe Journal of cell biology1996
Translocation of Src kinase to the cell periphery is mediated by the actin cytoskeleton under the control of the Rho family of small G proteins.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 178 citations in OpenAlex.
- Central role of protein kinase Cepsilon in constitutive activation of ERK1/2 and Rac1 in the malignant cells of hairy cell leukemia.The American journal of pathology · 2007Trial
- Article
- Promotion of Cx26 mutants located in TM4 region for membrane translocation successfully rescued hearing loss.Theranostics · 2025Article
- Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src.Cell death & disease · 2021Article
- Regulation of Fibroblast Cell Polarity by Src Tyrosine Kinase.Biomedicines · 2021Article
- N-terminus-independent activation of c-Src via binding to a tetraspan(in) TM4SF5 in hepatocellular carcinoma is abolished by the TM4SF5 C-terminal peptide application.Theranostics · 2021Article
- Historical retrospective of theMolecular and clinical oncology · 2020Review
- The mechano-sensitive response of β1 integrin promotes SRC-positive late endosome recycling and activation of Yes-associated protein.The Journal of biological chemistry · 2020Article
- CBFrontiers in cellular neuroscience · 2020Article
- The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover.The Journal of cell biology · 2019Article
- Mechanistic Insights into the Anti-angiogenic Activity of Trypanosoma cruzi Protein 21 and its Potential Impact on the Onset of Chagasic Cardiomyopathy.Scientific reports · 2017Article
- HLJ1 is an endogenous Src inhibitor suppressing cancer progression through dual mechanisms.Oncogene · 2016Article
- Src tyrosine kinase regulates the stem cell factor-induced breakdown of the blood-retinal barrier.Molecular vision · 2016Article
- Subcellular and Dynamic Coordination between Src Activity and Cell Protrusion in Microenvironment.Scientific reports · 2015Article
- Tropomyosin Tm5NM1 spatially restricts src kinase activity through perturbation of Rab11 vesicle trafficking.Molecular and cellular biology · 2014Article
- RhoA and membrane fluidity mediates the spatially polarized Src/FAK activation in response to shear stress.Scientific reports · 2014Article
- Serum starvation regulates E-cadherin upregulation via activation of c-Src in non-small-cell lung cancer A549 cells.American journal of physiology. Cell physiology · 2014Article
- Guiding brain tumor resection using surface-enhanced Raman scattering nanoparticles and a hand-held Raman scanner.ACS nano · 2014Article
- Engineered kinase activation reveals unique morphodynamic phenotypes and associated trafficking for Src family isoforms.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Endocytosis and the Src family of non-receptor tyrosine kinases.Biomolecular concepts · 2014Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated Swiss 3T3 subclones that are resistant to the mitogenic and morphological transforming effects of v-Src as a consequence of aberrant translocation of the oncoprotein under low serum conditions. In chicken embryo and NIH 3T3 fibroblasts under similar conditions, v-Src rapidly translocates from the perinuclear region to the focal adhesions upon activation of the tyrosine kinase, resulting in downstream activation of activator protein-1 and mitogen-activated protein kinase, which are required for the mitogenic and transforming activity of the oncoprotein. Since serum deprivation induces cytoskeletal disorganization in Swiss 3T3, we examined whether regulators of the cytoskeleton play a role in the translocation of v-Src, and also c-Src, in response to biological stimuli. Actin stress fibers and translocation of active v-Src to focal adhesions in quiescent Swiss 3T3 cells were restored by microinjection of activated Rho A and by serum. Double labeling with anti-Src and phalloidin demonstrated that v-Src localized along the reformed actin filaments in a pattern that would be consistent with trafficking in complexes along the stress fibers to focal adhesions. Furthermore, treatment with the actin-disrupting drug cytochalasin D, but not the microtubule-disrupting drug nocodazole, prevented v-Src translocation. In addition to v-Src, we observed that PDGF-induced, Rac-mediated membrane ruffling was accompanied by translocation of c-Src from the cytoplasm to the plasma membrane, an effect that was also blocked by cytochalasin D. Thus, we conclude that translocation of Src from its site of synthesis to its site of action at the cell membrane requires an intact cytoskeletal network and that the small G proteins of the Rho family may specify the peripheral localization in focal adhesions or along the membrane, mediated by their effects on the cytoskeleton.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.