ArticleThe Journal of cell biology1996
alpha-Tubulin limits its own synthesis: evidence for a mechanism involving translational repression.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Bridging the Gap: The Importance of TUBA1A α-Tubulin in Forming Midline Commissures.Frontiers in cell and developmental biology · 2021Article
- Differential requirements of tubulin genes in mammalian forebrain development.PLoS genetics · 2019Article
- Divergent microtubule assembly rates after short- versus long-term loss of end-modulating kinesins.Molecular biology of the cell · 2016Article
- Characterization and putative post-translational regulation of α- and β-tubulin gene families in Salix arbutifolia.Scientific reports · 2016Article
- Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus.Journal of experimental botany · 2015Article
- Random mutagenesis of β-tubulin defines a set of dispersed mutations that confer paclitaxel resistance.Pharmaceutical research · 2012Article
- Disruption of microtubule integrity initiates mitosis during CNS repair.Developmental cell · 2012Article
- Functional characterisation and drug target validation of a mitotic kinesin-13 in Trypanosoma brucei.PLoS pathogens · 2010Article
- How kinesin motor proteins drive mitotic spindle function: Lessons from molecular assays.Seminars in cell & developmental biology · 2010Review
- Regulation of Na,K-ATPase subunit abundance by translational repression.The Journal of biological chemistry · 2009Article
- Global regulation of the interphase microtubule system by abundantly expressed Op18/stathmin.Molecular biology of the cell · 2008Article
- The roles of beta-tubulin mutations and isotype expression in acquired drug resistance.Cancer informatics · 2007Article
- In vitro and in vivo analysis of microtubule-destabilizing kinesins.Methods in molecular biology (Clifton, N.J.) · 2007Article
- An unbiased cDNA library prepared from isolated Aplysia sensory neuron processes is enriched for cytoskeletal and translational mRNAs.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- EIF3 p170, a mediator of mimosine effect on protein synthesis and cell cycle progression.Molecular biology of the cell · 2003Article
- Cytochrome f translation in Chlamydomonas chloroplast is autoregulated by its carboxyl-terminal domain.The Plant cell · 2003Article
- Beta class II tubulin predominates in normal and tumor breast tissues.Breast cancer research : BCR · 2003Article
- K-loop insertion restores microtubule depolymerizing activity of a "neckless" MCAK mutant.The Journal of cell biology · 2002Article
- Plant tubulins: a melting pot for basic questions and promising applications.Transgenic research · 2000Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A Chinese hamster alpha-tubulin cDNA was modified to encode an 11-amino acid carboxyl-terminal extension containing the immunodominant epitope from influenza hemagglutinin antigen (to create HA alpha 1-tubulin) and was cloned into a vector for expression in mammalian cells. 12 stable CHO cell lines expressing this HA alpha 1-tubulin were isolated and characterized. HA alpha 1-tubulin incorporated into all classes of microtubules, assembled to the same extent as the endogenous tubulin, and did not perturb the growth of the cells in which it was expressed. However, overexpression of HA alpha 1-tubulin strongly repressed the synthesis of endogenous alpha-tubulin while having little or no effect on the synthesis of beta-tubulin. Treatment of transfected cells with sodium butyrate to induce even greater expression of HA alpha 1-tubulin led to a further decrease in synthesis of endogenous alpha-tubulin that was fully reversible upon removal of the inducer. Decreased synthesis of alpha-tubulin in transfected cells did not result from decreased levels of alpha-tubulin mRNA, as demonstrated by ribonuclease protection assays. On the other hand, colchicine, a drug previously shown to destabilize the tubulin message, caused a clear reduction in both protein synthesis and mRNA levels for transfected HA alpha 1-tubulin and endogenous alpha-tubulin, thus indicating that the decreased alpha-tubulin synthesis observed as a result of HA alpha 1-tubulin overexpression is distinct from the previously described autoregulation of tubulin. The results are consistent with a mechanism in which free alpha-tubulin inhibits the translation of its own message as a way of ensuring stoichiometric synthesis of alpha- and beta-tubulin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.