ArticleThe Journal of cell biology1996
Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 307 citations in OpenAlex.
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- The Membrane Phase Transition Gives Rise to Responsive Plasma Membrane Structure and Function.Cold Spring Harbor perspectives in biology · 2023Review
- Pre-organized landscape of T cell surface.Frontiers in immunology · 2023Review
- Coordinating Cytoskeleton and Molecular Traffic in T Cell Migration, Activation, and Effector Functions.Frontiers in cell and developmental biology · 2020Review
- O-glycans direct selectin ligands to lipid rafts on leukocytes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Multimodality imaging of coiled-coil mediated self-assembly in a "drug-free" therapeutic system.Advanced healthcare materials · 2015Article
- CD28 sensitizes TCR Ca²⁺ signaling during Ag-independent polarization of plasma membrane rafts.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Cytoskeletal modulation of lipid interactions regulates Lck kinase activity.The Journal of biological chemistry · 2012Article
- How does the kinase Lck phosphorylate the T cell receptor? Spatial organization as a regulatory mechanism.Frontiers in immunology · 2012Article
- A mechanistic model of early FcεRI signaling: lipid rafts and the question of protection from dephosphorylation.PloS one · 2012Article
- Inhibition of lymphoid tyrosine phosphatase by benzofuran salicylic acids.Journal of medicinal chemistry · 2011Article
- Control of genetically prescribed protein tyrosine kinase activities by environment-linked redox reactions.Enzyme research · 2011Article
- Functional role of gangliotetraosylceramide in epithelial-to-mesenchymal transition process induced by hypoxia and by TGF-{beta}.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Phosphorylation of leukocyte PECAM and its association with detergent-resistant membranes regulate transendothelial migration.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Glucosylceramide synthase upregulates MDR1 expression in the regulation of cancer drug resistance through cSrc and beta-catenin signaling.Molecular cancer · 2010Article
- T cell signal regulation by the actin cytoskeleton.The Journal of biological chemistry · 2010Article
- Dynamic regulation of CD45 lateral mobility by the spectrin-ankyrin cytoskeleton of T cells.The Journal of biological chemistry · 2010Article
- Cytoskeleton-membrane interactions in membrane raft structure.Cellular and molecular life sciences : CMLS · 2009Review
- Compartmentalization of phosphatidylinositol 4,5-bisphosphate signaling evidenced using targeted phosphatases.The Journal of biological chemistry · 2008Article
- Have we become overly reliant on lipid rafts? Talking Point on the involvement of lipid rafts in T-cell activation.EMBO reports · 2008Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
p56lck (Lck) is a lymphoid-specific Src family tyrosine kinase that is critical for T-cell development and activation. Lck is also a membrane protein, and approximately half of the membrane-associated Lck is associated with a glycolipid-enriched membrane (GEM) fraction that is resistant to solubilization by Triton X-100 (TX-100). To compare the membrane-associated Lck present in the GEM and TX-100-soluble fractions of Jurkat cells, Lck from each fraction was immunoblotted with antibody to phosphotyrosine. Lck in the GEM fraction was found to be hyperphosphorylated on tyrosine, and this correlated with a lower kinase specific activity relative to the TX-100-soluble Lck. Peptide mapping and phosphatase diagests showed that the hyperphosphorylation and lower kinase activity of GEM-associated Lck was due to phosphorylation of the regulatory COOH-terminal Tyr505. In addition, we determined that the membrane-bound tyrosine phosphatase CD45 was absent from the GEM fraction. Cells lacking CD45 showed identical phosphorylation of Lck in GEM and TX-100-soluble membranes. We propose that the GEM fraction represents a specific membrane domain present in T-cells, and that the hyperphosphorylation of tyrosine and lower kinase activity of GEM-associated Lck is due to exclusion of CD45 from these domains. Lck associated with the GEM domains may therefore consitute a reservoir of enzyme that can be readily activated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.