ArticleThe Journal of cell biology1996
Formation of nascent secretory vesicles from the trans-Golgi network of endocrine cells is inhibited by tyrosine kinase and phosphatase inhibitors.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 66 citations in OpenAlex.
- ADAM10 Site-Dependent Biology: Keeping Control of a Pervasive Protease.International journal of molecular sciences · 2021Review
- The endosomal system of plants: charting new and familiar territories.Plant physiology · 2008Review
- Testing for endocytosis in plants.Protoplasma · 2005Review
- A caspase cleavage fragment of p115 induces fragmentation of the Golgi apparatus and apoptosis.The Journal of cell biology · 2002Article
- Phospholipase D2 is localized to the rims of the Golgi apparatus in mammalian cells.Molecular biology of the cell · 2002Article
- Tyrosine-phosphorylated extracellular signal--regulated kinase associates with the Golgi complex during G2/M phase of the cell cycle: evidence for regulation of Golgi structure.The Journal of cell biology · 2001Article
- Intracellular localization of phospholipase D1 in mammalian cells.Molecular biology of the cell · 2001Article
- Signaling mediated by the cytosolic domain of peptidylglycine alpha-amidating monooxygenase.Molecular biology of the cell · 2001Article
- Interaction of the type Ialpha PIPkinase with phospholipase D: a role for the local generation of phosphatidylinositol 4, 5-bisphosphate in the regulation of PLD2 activity.The EMBO journal · 2000Article
- Phosphorylation of the rat pancreatic bile-salt-dependent lipase by casein kinase II is essential for secretion.The Biochemical journal · 2000Article
- Identification of a new Pyk2 target protein with Arf-GAP activity.Molecular and cellular biology · 1999Article
- Regulation of endosome sorting by a specific PP2A isoform.The Journal of cell biology · 1998Article
- Biogenesis of secretory granules in the trans-Golgi network of neuroendocrine and endocrine cells.Biochimica et biophysica acta · 1998Review
- Tyrosine phosphorylation of selected secretory carrier membrane proteins, SCAMP1 and SCAMP3, and association with the EGF receptor.Molecular biology of the cell · 1998Article
- Sorting and storage during secretory granule biogenesis: looking backward and looking forward.The Biochemical journal · 1998Review
- Functional association between Arf and RalA in active phospholipase D complex.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Tetrahymena: the key to the genetic analysis of the regulated pathway of polypeptide secretion?Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Phospholipase D stimulates release of nascent secretory vesicles from the trans-Golgi network.The Journal of cell biology · 1997Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Recent evidence suggests that secretory vesicle formation from the TGN is regulated by cytosolic signaling pathways involving small GTP-binding proteins, heterotrimeric G proteins, inositol phospholipid metabolism, and protein serine/threonine phosphorylation. At the cell surface, protein phosphorylation and dephosphorylation on tyrosine residues can rapidly modulate cytosolic signaling pathways in response to extracellular stimuli and have been implicated in the internalization and sorting of signaling receptors. to determine if phosphotyrosine metabolism might also regulate secretory vesicle budding from the TGN, we treated permeabilized rat pituitary GH3 cells with inhibitors of either tyrosine phosphatases or tyrosine kinases. We demonstrate that the tyrosine phosphatase inhibitors pervanadate and zinc potently inhibited budding of nascent secretory vesicles. Tyrphostin A25 (TA25) and other tyrosine kinase inhibitors also prevented secretory vesicle release, suggesting that vesicle formation requires both phosphatase and kinase activities. A stimulatory peptide derived from the NH2 terminus of the small GTP-binding protein ADP ribosylation factor 1 (ARF1) antagonized the inhibitory effect of TA25, indicating that both agents influence the same pathway leading to secretory vesicle formation. Antiphosphotyrosine immunoblotting revealed that protein tyrosine phosphorylation was enhanced after treatment with tyrosine phosphatase or kinase inhibitors. Subcellular fractionation identified several tyrosine phosphorylated polypeptides of approximately 175, approximately 130, and 90-110 kD that were enriched in TGN-containing Golgi fractions and tightly membrane associated. The phosphorylation of these polypeptides correlated with inhibition of vesicle budding. Our results suggest that in endocrine cells, protein tyrosine phosphrylation and dephosphorylation are required for secretory vesicle release from the TGN.
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