ArticleThe EMBO journal1996
Heat shock disassembles the nucleolus and inhibits nuclear protein import and poly(A)+ RNA export.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 67 citations in OpenAlex.
- ENY2 transcription and export complex 2 subunit deficiency induces nucleolar stress to inhibit tumor progression through NPM1/MDM2/p53-dependent and -independent responses.Cellular oncology (Dordrecht, Netherlands) · 2026Article
- Fragmentation of nucleoli in senescent cancer cells is associated with increased levels of polyadenylated transcripts derived from noncoding regions of rDNA units.European biophysics journal : EBJ · 2025Article
- Translational regulation in stress biology.Nature cell biology · 2025Review
- Early rRNA processing is a stress-dependent regulatory event whose inhibition maintains nucleolar integrity.Nucleic acids research · 2022Article
- Phase Separation of Intrinsically Disordered Nucleolar Proteins Relate to Localization and Function.International journal of molecular sciences · 2021Review
- A zygote-based assay to evaluate intranuclear shuttling inSTAR protocols · 2021Article
- The nucleolus: a central response hub for the stressors that drive cancer progression.Cellular and molecular life sciences : CMLS · 2019Review
- A ribosome assembly stress response regulates transcription to maintain proteome homeostasis.eLife · 2019Article
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- Sequential RNA degradation pathways provide a fail-safe mechanism to limit the accumulation of unspliced transcripts in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2012Article
- Role of the heat shock transcription factor, Hsf1, in a major fungal pathogen that is obligately associated with warm-blooded animals.Molecular microbiology · 2009Article
- HSF1-TPR interaction facilitates export of stress-induced HSP70 mRNA.The Journal of biological chemistry · 2007Article
- The specialized cytosolic J-protein, Jjj1, functions in 60S ribosomal subunit biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Inhibition of nuclear import by the proapoptotic protein CC3.Molecular and cellular biology · 2004Article
- Disruption of the nucleolus mediates stabilization of p53 in response to DNA damage and other stresses.The EMBO journal · 2003Article
- Localization of nuclear retained mRNAs in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2003Article
- The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm.The EMBO journal · 2002Article
- Protein ligands mediate the CRM1-dependent export of HuR in response to heat shock.RNA (New York, N.Y.) · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat shock causes major positive and negative changes in gene expression, drastically alters the appearance of the nucleolus and inhibits rRNA synthesis. We here show that it causes many yeast nucleolar proteins, including the fibrillarin homolog Nop1p, to relocate to the cytoplasm. Relocation depends on several proteins implicated in mRNA transport (Mtrps) and is reversible. Two observations indicate, surprisingly, that disassembly results from a reduction in Ssa protein (Hsp70) levels: (i) selective depletion of Ssa1p leads to disassembly of the nucleolus; (ii) preincubation at 37 degrees C protects the nucleolus against disassembly by heat shock, unless expression of Ssa proteins is specifically inhibited. We observed that heat shock or reduction of Ssa1p levels inhibits protein import into the nucleus and therefore we propose that inhibition of import leads to disassembly of the nucleolus. These observations provide a simple explanation of the effects of heat shock on the anatomy of the nucleolus and rRNA transcription. They also extend understanding of the path of nuclear export. Since a number of nucleoplasmic proteins also relocate upon heat shock, these observations can provide a general mechanism for regulation of gene expression. Relocation of the hnRNP-like protein Mtr13p (= Npl3p, Nop3p), explains the heat shock sensitivity of export of average poly(A)+ RNA. Strikingly, Hsp mRNA export appears not to be affected.
Indexed as
Identifiers
8978700PMC452498W1715492285What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.