ArticleThe EMBO journal1996
A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 162 citations in OpenAlex.
- CDPK gene family in Camellia nitidissima: genome-wide identification and expression analysis during petal development and hormone treatments.BMC plant biology · 2026Article
- Impaired Function of the Calcium-Dependent Protein Kinase,Frontiers in plant science · 2019Article
- The Membrane Transport System of the Guard Cell and Its Integration for Stomatal Dynamics.Plant physiology · 2017Review
- Vacuolar ion channels in the liverwort Marchantia polymorpha: influence of ion channel inhibitors.Planta · 2017Article
- THI1, a Thiamine Thiazole Synthase, Interacts with Ca2+-Dependent Protein Kinase CPK33 and Modulates the S-Type Anion Channels and Stomatal Closure in Arabidopsis.Plant physiology · 2016Article
- Anion Channel Inhibitor NPPB-Inhibited Fluoride Accumulation in Tea Plant (Camellia sinensis) Is Related to the Regulation of Ca²⁺, CaM and Depolarization of Plasma Membrane Potential.International journal of molecular sciences · 2016Article
- Calcium Sensors as Key Hubs in Plant Responses to Biotic and Abiotic Stresses.Frontiers in plant science · 2016Review
- A vacuolar phosphate transporter essential for phosphate homeostasis in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Arabidopsis CALCIUM-DEPENDENT PROTEIN KINASE8 and CATALASE3 Function in Abscisic Acid-Mediated Signaling and H2O2 Homeostasis in Stomatal Guard Cells under Drought Stress.The Plant cell · 2015Article
- Article
- 14-3-3 Proteins in Guard Cell Signaling.Frontiers in plant science · 2015Review
- Multi-level modeling of light-induced stomatal opening offers new insights into its regulation by drought.PLoS computational biology · 2014Article
- Cross-talk between abscisic acid-dependent and abscisic acid-independent pathways during abiotic stress.Plant cell reports · 2013Review
- Overexpression of StDREB1 transcription factor increases tolerance to salt in transgenic potato plants.Molecular biotechnology · 2013Article
- The myristoylated amino-terminus of an Arabidopsis calcium-dependent protein kinase mediates plasma membrane localization.Plant molecular biology · 2013Article
- Ca2+-dependent protein kinase11 and 24 modulate the activity of the inward rectifying K+ channels in Arabidopsis pollen tubes.The Plant cell · 2013Article
- CDPKs in immune and stress signaling.Trends in plant science · 2013Review
- AtALMT9 is a malate-activated vacuolar chloride channel required for stomatal opening in Arabidopsis.Nature communications · 2013Article
- Expression of StMYB1R-1, a novel potato single MYB-like domain transcription factor, increases drought tolerance.Plant physiology · 2011Article
- Arabidopsis calcium-dependent protein kinase CPK10 functions in abscisic acid- and Ca2+-mediated stomatal regulation in response to drought stress.Plant physiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcium-Dependent Protein Kinases (CDPKs) in higher plants contain a C-terminal calmodulin-like regulatory domain. Little is known regarding physiological CDPK targets. Both kinase activity and multiple Ca2+-dependent signaling pathways have been implicated in the control of stomatal guard cell movements. To determine whether CDPK or other protein kinases could have a role in guard cell signaling, purified and recombinant kinases were applied to Vicia faba guard cell vacuoles during patch-clamp experiments. CDPK activated novel vacuolar chloride (VCL) and malate conductances in guard cells. Activation was dependent on both Ca2+ and ATP. Furthermore, VCL activation occurred in the absence of Ca2+ using a Ca2+-independent, constitutively active, CDPK* mutant. Protein kinase A showed weaker activation (22% as compared with CDPK). Current reversals in whole vacuole recordings shifted with the Nernst potential for Cl-and vanished in glutamate. Single channel recordings showed a CDPK-activated 34 +/- 5 pS Cl- channel. VCL channels were activated at physiological potentials enabling Cl- uptake into vacuoles. VCL channels may provide a previously unidentified, but necessary, pathway for anion uptake into vacuoles required for stomatal opening. CDPK-activated VCL currents were also observed in red beet vacuoles suggesting that these channels may provide a more general mechanism for kinase-dependent anion uptake.
Indexed as
Identifiers
8978683PMC452481W144778061What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.