ArticleGenetics1996
Functional and genetic characterization of the oligomerization and DNA binding properties of the Drosophila doublesex proteins.
Article in Genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 105 citations in OpenAlex.
- Understanding the genetics of sex determination in insects and its relevance to genetic pest management.Insect molecular biology · 2025Review
- Challenges in developing a split drive targeting dsx for the genetic control of the invasive malaria vector Anopheles stephensi.Parasites & vectors · 2025Article
- Candidate target genes of the male-specific expressed Doublesex in the termite Reticulitermes speratus.PloS one · 2024Article
- Evolutionary History of Sexual Differentiation Mechanism in Insects.Molecular biology and evolution · 2022Article
- Regulation of Doublesex1 Expression for Environmental Sex Determination in the Cladoceran Crustacean Daphnia.Frontiers in cell and developmental biology · 2022Review
- DMRT Transcription Factors in the Control of Nervous System Sexual Differentiation.Frontiers in neuroanatomy · 2022Review
- Hemimetabolous insects elucidate the origin of sexual development via alternative splicing.eLife · 2019Article
- Precise staging of beetle horn formation in Trypoxylus dichotomus reveals the pleiotropic roles of doublesex depending on the spatiotemporal developmental contexts.PLoS genetics · 2019Article
- Doublesex Evolution Is Correlated with Social Complexity in Ants.Genome biology and evolution · 2018Article
- Haploinsufficiency of theBalkan medical journal · 2018Article
- The regulatory repertoire of PLZF and SALL4 in undifferentiated spermatogonia.Development (Cambridge, England) · 2016Article
- Sex-specific regulation of Lgr3 in Drosophila neurons.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Double nexus--Doublesex is the connecting element in sex determination.Briefings in functional genomics · 2015Review
- The Wright stuff: reimagining path analysis reveals novel components of the sex determination hierarchy in Drosophila melanogaster.BMC systems biology · 2015Article
- Rare double sex and mab-3-related transcription factor 1 regulatory variants in severe spermatogenic failure.Andrology · 2015Article
- The ubiquity and ancestry of insect doublesex.Scientific reports · 2015Article
- Sex- and tissue-specific functions of Drosophila doublesex transcription factor target genes.Developmental cell · 2014Article
- Developmental link between sex and nutrition; doublesex regulates sex-specific mandible growth via juvenile hormone signaling in stag beetles.PLoS genetics · 2014Article
- Tribolium castaneum Transformer-2 regulates sex determination and development in both males and females.Insect biochemistry and molecular biology · 2013Article
- Expanding roles for the evolutionarily conserved Dmrt sex transcriptional regulators during embryogenesis.Cellular and molecular life sciences : CMLS · 2013Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The doublesex (dsx) gene of Drosophila melanogaster encodes both male-specific (DSXM) and female-specific (DSXF) polypeptides, which are required for normal differentiation of numerous sexually dimorphic somatic traits. The DSX polypeptides are transcription factors and have been shown previously to bind through a zinc finger-like domain to specific sites in an enhancer regulating sex-specific expression of yolk protein genes. We have determined the consensus target sequence for this DNA binding domain to be a palindromic sequence AGNNACTAAATGTNNTC composed of two half-sites around a central (A/T) base pair. As predicted by the symmetric nature of this site, we have found that the DSX proteins exist as dimers in vivo and have mapped two independent dimerization domains by the yeast two-hybrid method; one in the non-sex-specific amino-terminal region of the protein and one that includes the partially sex-specific carboxy-terminal domains of both the male and female polypeptides. We have further identified a missense mutation that eliminates dsx function in female flies, and shown that the same mutation prevents dimerization of DSXF in the yeast two-hybrid system, indicating a critical role for dimerization in dsx function in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.