ArticleInfection and immunity1997
Development of an in vitro macrophage system to assess Penicillium marneffei growth and susceptibility to nitric oxide.
Article in Infection and immunity, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Rapid diagnosis ofFrontiers in cellular and infection microbiology · 2022Pooled it
- Glutathione Peroxidase fromInternational journal of molecular sciences · 2026Article
- Differential innate immune responses of human macrophages and bronchial epithelial cells againstmSphere · 2023Article
- Talaromyces marneffei suppresses macrophage inflammation by regulating host alternative splicing.Communications biology · 2023Article
- Exosomes released from macrophages infected with Talaromyces marneffei activate the innate immune responses and decrease the replication.Immunity, inflammation and disease · 2023Article
- An Overlooked and Underrated Endemic Mycosis-Talaromycosis and the Pathogenic Fungus Talaromyces marneffei.Clinical microbiology reviews · 2023Review
- Overexpression of CD86 enhances the ability of THP-1 macrophages to defend against Talaromyces marneffei.Immunity, inflammation and disease · 2022Article
- Review
- Role and mechanism of the Dectin-1-mediated Syk/NF-κB signaling pathway inExperimental and therapeutic medicine · 2022Article
- Article
- Extracellular Vesicles Derived FromFrontiers in microbiology · 2020Article
- Macrophages protect Talaromyces marneffei conidia from myeloperoxidase-dependent neutrophil fungicidal activity during infection establishment in vivo.PLoS pathogens · 2018Article
- Adaptation to macrophage killing byFuture science OA · 2017Review
- Article
- Evaluation of antibacterial and cytotoxic activity of Artemisia nilagirica and Murraya koenigii leaf extracts against mycobacteria and macrophages.BMC complementary and alternative medicine · 2014Article
- Development of in vitro macrophage system to evaluate phagocytosis and intracellular fate of Penicillium marneffei conidia.Mycopathologia · 2013Article
- Penicillium marneffei infection and recent advances in the epidemiology and molecular biology aspects.Clinical microbiology reviews · 2006Review
- Differential cytokine pattern in the spleens and livers of BALB/c mice infected with Penicillium marneffei: protective role of gamma interferon.Infection and immunity · 2003Article
- Inhibition of intramacrophage growth of Penicillium marneffei by 4-aminoquinolines.Antimicrobial agents and chemotherapy · 2001Article
- Nitric oxide participation in the fungicidal mechanism of gamma interferon-activated murine macrophages against Paracoccidioides brasiliensis conidia.Infection and immunity · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the effect of nitric oxide (NO) and reactive nitrogen intermediates on the in vitro growth of Penicillium marneffei both in a cell-free system and in a novel macrophage culture system. In the cell-free system, NO that was chemically generated from NaNO2 in acid media (pH 4 and 5) markedly inhibited the growth of P. marneffei. On the contrary, inhibition of growth did not occur in neutral medium (pH 7.4) in which NO was not produced. P. marneffei conidia were phagocytized by nonstimulated murine J774 macrophages after 2 h of incubation. During the following 24 h, P. marneffei grew as yeast-like cells replicating by fission in the J774 macrophages. The intracellular growth of P. marneffei damaged nonstimulated J774 macrophages, as confirmed by electron microscopy. When J774 cells were stimulated by gamma interferon and lipopolysaccharide, which led to enhanced production of reactive nitrogen intermediates, the percentage of yeast-like cells was significantly reduced and P. marneffei conidia were damaged in the J774 macrophages. The inhibition of NO synthesis by N-monomethyl-L-arginine restored the intracellular growth of P. marneffei. The inverse correlation between intramacrophage growth and the amount of nitrite detected in culture supernatants supports the hypothesis that the L-arginine-dependent NO pathway plays an important role in the murine macrophage immune response against P. marneffei.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.