ArticleApplied and environmental microbiology1996
Expression of a Trichoderma reesei beta-xylanase gene (XYN2) in Saccharomyces cerevisiae.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 146 citations in OpenAlex.
- Metabolic recycling of storage lipids promotes squalene biosynthesis in yeast.Biotechnology for biofuels and bioproducts · 2022Article
- Cloning, expression, and characterization of a recombinant xylanase from Bacillus sonorensis T6.PloS one · 2022Article
- Xylo-Oligosaccharide Utilization by EngineeredFrontiers in bioengineering and biotechnology · 2022Review
- Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris.BMC biotechnology · 2021Article
- Characterizing a Halo-Tolerant GH10 Xylanase fromInternational journal of molecular sciences · 2019Article
- Efficient Expression of Xylanase by Codon Optimization and Its Effects on the Growth Performance and Carcass Characteristics of Broiler.Animals : an open access journal from MDPI · 2019Article
- Engineering of Saccharomyces cerevisiae to utilize xylan as a sole carbohydrate source by co-expression of an endoxylanase, xylosidase and a bacterial xylose isomerase.Journal of industrial microbiology & biotechnology · 2016Article
- Expression and evaluation of enzymes required for the hydrolysis of galactomannan.Journal of industrial microbiology & biotechnology · 2014Article
- Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases.Biotechnology for biofuels · 2013Article
- Identification of an endo-1,4-beta-xylanase of Ustilago maydis.BMC biotechnology · 2013Article
- Fungal β-glucosidase expression in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2012Article
- Achieving efficient protein expression in Trichoderma reesei by using strong constitutive promoters.Microbial cell factories · 2012Article
- Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome.Applied and environmental microbiology · 2012Article
- Biorefining of wood: combined production of ethanol and xylanase from waste fiber sludge.Journal of industrial microbiology & biotechnology · 2011Article
- Expression of endo-1, 4-beta-xylanase from Trichoderma reesei in Pichia pastoris and functional characterization of the produced enzyme.BMC biotechnology · 2009Article
- Strain improvement of Trichoderma reesei Rut C-30 for increased cellulase production.Indian journal of microbiology · 2009Article
- Production of the Aspergillus aculeatus endo-1,4-beta-mannanase in A. niger.Journal of industrial microbiology & biotechnology · 2009Article
- Controlled expression of the dominant flocculation genes FLO1, FLO5, and FLO11 in Saccharomyces cerevisiae.Applied and environmental microbiology · 2008Article
- Role of cultivation media in the development of yeast strains for large scale industrial use.Microbial cell factories · 2005Article
- Construction of a xylan-fermenting yeast strain through codisplay of xylanolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells.Applied and environmental microbiology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The XYN2 gene encoding the main Trichoderma reesei QM 6a endo-beta-1,4-xylanase was amplified by PCR from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 699-bp open reading frame that encodes a 223-amino-acid propeptide. The XYN2 gene, located on URA3-based multicopy shuttle vectors, was successfully expressed in the yeast Saccharomyces cerevisiae under the control of the alcohol dehydrogenase II (ADH2) and phosphoglycerate kinase (PGK1) gene promoters and terminators, respectively. The 33-amino-acid leader peptide of the Xyn2 beta-xylanase was recognized and cleaved at the Kex2-like Lys-Arg residues, enabling the efficient secretion and glycosylation of the heterologous beta-xylanase. The molecular mass of the recombinant beta-xylanase was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 27 kDa. The construction of fur1 ura3 S. cerevisiae strains allowed for the autoselection of the URA3-based XYN2 shuttle vectors in nonselective complex medium. These autoselective S. cerevisiae strains produced 1,200 and 160 nkat of beta-xylanase activity per ml under the control of the ADH2 and PGK1 promoters in rich medium, respectively. The recombinant enzyme showed highest activity at pH 6 and 60 degrees C and retained more than 90% of its activity after 60 min at 50 degrees C.
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