ArticleClinical rheumatology1996
Intermittent compressive load stimulates osteogenesis and improves osteocyte viability in bones cultured "in vitro".
Article in Clinical rheumatology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 47 citations in OpenAlex.
- Culture system for longitudinal monitoring of bone dynamics ex vivo.Biotechnology and bioengineering · 2025Article
- The Osteocyte: From "Prisoner" to "Orchestrator".Journal of functional morphology and kinesiology · 2021Review
- Ex vivo Bone Models and Their Potential in Preclinical Evaluation.Current osteoporosis reports · 2021Review
- Expression and functional proteomic analyses of osteocytes from Xenopus laevis tested under mechanical stress conditions: preliminary observations on an appropriate new animal model.Journal of anatomy · 2017Article
- Shifting paradigms on the role of connexin43 in the skeletal response to mechanical load.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Review
- The treatment of medial tibial stress syndrome in athletes; a randomized clinical trial.Sports medicine, arthroscopy, rehabilitation, therapy & technology : SMARTT · 2012Article
- Enhanced osteoclastic resorption and responsiveness to mechanical load in gap junction deficient bone.PloS one · 2011Article
- Gap Junctions and Biophysical Regulation of Bone Cells.Clinical reviews in bone and mineral metabolism · 2010Article
- Regenerate healing outcomes in unilateral mandibular distraction osteogenesis using quantitative histomorphometry.Plastic and reconstructive surgery · 2010Article
- The influence of mechanical stimulation on osteocyte apoptosis and bone viability in human trabecular bone.Journal of musculoskeletal & neuronal interactionsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of mechanical stresses on osteogenesis, the viability of osteocytes and their metabolic activity in organ culture of bones intermittently loaded "in vitro" are reported. Metatarsal bones, isolated from 12-day-old rats, were cultured in BGJb medium (with 10% foetal calf serum, 75 micrograms/ml of ascorbic acid, 100 U/ml of penicillin and 100 micrograms/ml of streptomycin), in humidified air enriched by 5% CO2 and 30% O2, and loaded in our original device for 1/2 an hour at 1 Hz. homotypic isolated and unloaded bones, cultured in the same medium, were taken as controls. The ALP (alkaline phophatase activity) increases in the media of loaded bones in comparison with the control bones. The percentage of viable osteocytes is significantly greater in loaded than in control bones. TEM observations demonstrate that in both loaded and control unloaded bones, osteocytes show well developed organelle machinery and several gap junctions with adjacent cellular processes. In the cells of loaded bones, however, a higher number of cytoplasmic organelles and gap junctions were found. In particular, RER increases twice, gap junctions three times. The induced osteogenesis and the TEM observations demonstrate the suitability of this experimental model and support the recent advanced hypothesis according to which the mechanical loading may exert a trophic function on osteocytes, stimulating both the proteic synthesis in the above-mentioned cells and the cell-to-cell communication. Furthermore, the loading is likely to exert a biological stimulus on osteoblasts via signalling molecules produced by osteocytes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.