ArticleThe Biochemical journal1996
Role of P2-purinergic receptors in rat Leydig cell steroidogenesis.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Enokitake Mushroom and Its Active Component, Adenosine, Which Restores Testosterone Production in Impaired and Fatigued Mouse Models.Nutrients · 2023Article
- Molecular and Pharmacological Evidence for the Expression of Multiple Functional P2 Purinergic Receptors in Human Adipocytes.Molecules (Basel, Switzerland) · 2022Article
- Purinergic signaling in the male reproductive tract.Frontiers in endocrinology · 2022Review
- Article
- Sex-Related Overactivation of NLRP3 Inflammasome Increases Lethality of the Male COVID-19 Patients.Frontiers in molecular biosciences · 2021Review
- Association of G-protein coupled purinergic receptor P2Y2 with ischemic stroke in a Han Chinese population of North China.Neural regeneration research · 2019Article
- Nitric oxide modulates ATP-evoked currents in mouse Leydig cells.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2018Article
- Distinct purinergic signaling pathways in prepubescent mouse spermatogonia.The Journal of general physiology · 2016Article
- Purinergic signaling in testes revealed.The Journal of general physiology · 2016Article
- Extracellular ATP and other nucleotides-ubiquitous triggers of intercellular messenger release.Purinergic signalling · 2016Review
- Purinergic signaling pathways in endocrine system.Autonomic neuroscience : basic & clinical · 2015Review
- Purinergic signalling in endocrine organs.Purinergic signalling · 2014Review
- Purinergic signalling in the reproductive system in health and disease.Purinergic signalling · 2014Review
- P2X receptor channels in endocrine glands.Wiley interdisciplinary reviews. Membrane transport and signaling · 2013Article
- ATP secretion in the male reproductive tract: essential role of CFTR.The Journal of physiology · 2012Article
- Interaction of purinergic receptors with GPCRs, ion channels, tyrosine kinase and steroid hormone receptors orchestrates cell function.Purinergic signalling · 2012Article
- High expression and activity of ecto-5'-nucleotidase/CD73 in the male murine reproductive tract.Histochemistry and cell biology · 2010Article
- The purinergic receptor P2Y, G-protein coupled, 2 (P2RY2) gene associated with essential hypertension in Japanese men.Journal of human hypertension · 2010Article
- Mouse Leydig cells express multiple P2X receptor subunits.Purinergic signalling · 2009Article
- Localization of plasma membrane bound NTPDases in the murine reproductive tract.Histochemistry and cell biology · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study investigated the effects of extracellular ATP on the intracellular calcium ion concentration ([Ca2+]i) and testosterone production in isolated adult rat Leydig cells. This nucleotide caused an increase in [Ca2+]i, with a maximal effect at a concentration of 100 microM ATP, comprising a rapid initial spike followed by a long-lasting plateau. The first rapid spike was dependent on the release of Ca2+ from internal stores, since it also occurred in Ca(2+)-free medium and was abolished after depletion of internal stores with thapsigargin. The second, long-lasting, phase was dependent on the influx of Ca2+ from the extracellular medium. After 3 h of incubation, extracellular ATP stimulated testosterone secretion in a dose-dependent manner, with a maximal effect at 100 microM. Activation of steroidogenesis by ATP was fully dependent on the presence of Ca2+ in the external medium. Among different nucleotides, only ATP, adenosine 5'-[gamma-thio]triphosphate, UTP, benzoylbenzoic-ATP and 2-methylthio-ATP were effective in inducing both the rise in [Ca2+]i and testosterone secretion. These effects were blocked by preincubation of Leydig cells with oxidized ATP, an inhibitor of the P2Z-purinergic receptor subtype. These results show that rat Leydig cells possess P2-purinergic receptors whose activation triggers an increase in [Ca2+]i due to the release of Ca2+ from internal stores and Ca2+ influx from the external medium. The stimulatory effect of extracellular ATP on testosterone secretion seems to be coupled to the influx of Ca2+ from the external medium.
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