ArticleNucleic acids research1996
Species-specificity of rRNA gene transcription in plants manifested as a switch in RNA polymerase specificity.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- HSF1 induces RNA polymerase II synthesis of ribosomal RNA in S. cerevisiae during nitrogen deprivation.Current genetics · 2021Article
- Development of a Virus-Based Reporter System for Functional Analysis of Plant rRNA Gene Promoter.Frontiers in microbiology · 2021Article
- Ribosome Biogenesis in Plants: From Functional 45S Ribosomal DNA Organization to Ribosome Assembly Factors.The Plant cell · 2019Review
- Alternative reverse genetics system for influenza viruses based on a synthesized swine 45S rRNA promoter.Virus genes · 2017Article
- Comparative genetic analysis of the 45S rDNA intergenic spacers from three Saccharum species.PloS one · 2017Article
- Cloning the Horse RNA Polymerase I Promoter and Its Application to Studying Influenza Virus Polymerase Activity.Viruses · 2016Article
- The plant-specific TFIIB-related protein, pBrp, is a general transcription factor for RNA polymerase I.The EMBO journal · 2008Article
- Sex-biased lethality or transmission of defective transcription machinery in Arabidopsis.Genetics · 2008Article
- Article
- Organization, differential expression and methylation of rDNA in artificial Solanum allopolyploids.Plant molecular biology · 2004Article
- Transcription and tyranny in the nucleolus: the organization, activation, dominance and repression of ribosomal RNA genes.The arabidopsis book · 2002Article
- Transcription of chromosomal rRNA genes by both RNA polymerase I and II in yeast uaf30 mutants lacking the 30 kDa subunit of transcription factor UAF.The EMBO journal · 2001Article
- Regulation of plastid rDNA transcription by interaction of CDF2 with two different RNA polymerases.The EMBO journal · 2000Article
- Survey and summary: transcription by RNA polymerases I and III.Nucleic acids research · 2000Review
- Article
- RNA polymerase switch in transcription of yeast rDNA: role of transcription factor UAF (upstream activation factor) in silencing rDNA transcription by RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Extensive purification of a putative RNA polymerase I holoenzyme from plants that accurately initiates rRNA gene transcription in vitro.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Epigenetic silencing of RNA polymerase I transcription: a role for DNA methylation and histone modification in nucleolar dominance.Genes & development · 1997Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Rapid evolution of ribosomal RNA (rRNA) gene promoters often prevents their recognition in a foreign species. Unlike animal systems, we show that foreign plant rRNA gene promoters are recognized in an alien species, but tend to program transcription by a different polymerase. In plants, RNA polymerase I transcripts initiate at a TATATA element (+1 is underlined) important for promoter strength and start-site selection. However, transcripts initiate from +32 following transfection of a tomato promoter into Arabidopsis. The rRNA gene promoter of a more closely related species, Brassica oleracea, programs both +1 and +29 transcription. A point mutation at +2 improving the identity between the Brassica and Arabidopsis promoters increases +1 transcription, indicating a role for the initiator element in species-specificity. Brassica +29 transcripts can be translated to express a luciferase reporter gene, implicating RNA polymerase II. TATA mutations that disrupt TATA-binding protein (TBP) interactions inhibit +29 transcription and luciferase expression. Co-expressed TBP proteins bearing compensatory mutations restore +29 transcription and luciferase activity, suggesting a direct TBP-TATA interaction. Importantly, +1 transcription is unaffected by the TATA mutations, suggesting that in the context of pol I recognition, the TATA-containing initiator element serves a function other than TBP binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.