ArticleMolecular and cellular biology1997
Active mammalian replication origins are associated with a high-density cluster of mCpG dinucleotides.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 96 citations in OpenAlex.
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- Impact of brown rice-specific γ-oryzanol on epigenetic modulation of dopamine D2 receptors in brain striatum in high-fat-diet-induced obesity in mice.Diabetologia · 2017Article
- DNA sequence templates adjacent nucleosome and ORC sites at gene amplification origins in Drosophila.Nucleic acids research · 2015Article
- Differential chromatin structure encompassing replication origins in transformed and normal cells.Genes & cancer · 2012Article
- Late-replicating heterochromatin is characterized by decreased cytosine methylation in the human genome.Genome research · 2011Article
- DNA methylation detection: bisulfite genomic sequencing analysis.Methods in molecular biology (Clifton, N.J.) · 2011Article
- A modified protocol for bisulfite genomic sequencing of difficult samples.Biological procedures online · 2009Article
- Cancer testis antigens in human melanoma stem cells: expression, distribution, and methylation status.Journal of cellular physiology · 2008Article
- Circuit assemblages derived from net dinucleotide values provide a succinct identity for the HIV-1 genome and each of its genes.Virus genes · 2008Article
- MSRE-PCR for analysis of gene-specific DNA methylation.Nucleic acids research · 2005Article
- DNA methylation may restrict but does not determine differential gene expression at the Sgy/Tead2 locus during mouse development.Molecular and cellular biology · 2004Article
- Non-methylated Genomic Sites Coincidence Cloning (NGSCC): an approach to large scale analysis of hypomethylated CpG patterns at predetermined genomic loci.Molecular genetics and genomics : MGG · 2004Article
- Methylation patterns of papillomavirus DNA, its influence on E2 function, and implications in viral infection.Journal of virology · 2003Article
- Replication of the chicken beta-globin locus: early-firing origins at the 5' HS4 insulator and the rho- and betaA-globin genes show opposite epigenetic modifications.Molecular and cellular biology · 2003Article
- Fidelity of the methylation pattern and its variation in the genome.Genome research · 2003Article
- Article
- Cloning of the 5' upstream region of the rat p16 gene and its role in silencing.Japanese journal of cancer research : Gann · 2002Article
- Methylome profiling of cancer cells by amplification of inter-methylated sites (AIMS).Nucleic acids research · 2002Article
- Article
- Enhanced flexibility and aphidicolin-induced DNA breaks near mammalian replication origins: implications for replicon mapping and chromosome fragility.Nucleic acids research · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ori-beta is a well-characterized origin of bidirectional replication (OBR) located approximately 17 kb downstream of the dihydrofolate reductase gene in hamster cell chromosomes. The approximately 2-kb region of ori-beta that exhibits greatest replication initiation activity also contains 12 potential methylation sites in the form of CpG dinucleotides. To ascertain whether DNA methylation might play a role at mammalian replication origins, the methylation status of these sites was examined with bisulfite to chemically distinguish cytosine (C) from 5-methylcytosine (mC). All of the CpGs were methylated, and nine of them were located within 356 bp flanking the minimal OBR, creating a high-density cluster of mCpGs that was approximately 10 times greater than average for human DNA. However, the previously reported densely methylated island in which all cytosines were methylated regardless of their dinucleotide composition was not detected and appeared to be an experimental artifact. A second OBR, located at the 5' end of the RPS14 gene, exhibited a strikingly similar methylation pattern, and the organization of CpG dinucleotides at other mammalian origins revealed the potential for high-density CpG methylation. Moreover, analysis of bromodeoxyuridine-labeled nascent DNA confirmed that active replication origins were methylated. These results suggest that a high-density cluster of mCpG dinucleotides may play a role in either the establishment or the regulation of mammalian replication origins.
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