ArticleMolecular and cellular biology1997
Evidence that Spt3 functionally interacts with Mot1, TFIIA, and TATA-binding protein to confer promoter-specific transcriptional control in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 103 citations in OpenAlex.
- Spt3 and Spt8 Are Involved in the Formation of a Silencing Boundary by Interacting with TATA-Binding Protein.Biomolecules · 2023Article
- Promoter-dependent nuclear RNA degradation ensures cell cycle-specific gene expression.Communications biology · 2019Article
- Improvement of Lead Tolerance of Saccharomyces cerevisiae by Random Mutagenesis of Transcription Regulator SPT3.Applied biochemistry and biotechnology · 2018Article
- The Modifier of Transcription 1 (Mot1) ATPase and Spt16 Histone Chaperone Co-regulate Transcription through Preinitiation Complex Assembly and Nucleosome Organization.The Journal of biological chemistry · 2016Article
- Mot1 redistributes TBP from TATA-containing to TATA-less promoters.Molecular and cellular biology · 2013Article
- Direct TFIIA-TFIID protein contacts drive budding yeast ribosomal protein gene transcription.The Journal of biological chemistry · 2013Article
- Tight cooperation between Mot1p and NC2β in regulating genome-wide transcription, repression of transcription following heat shock induction and genetic interaction with SAGA.Nucleic acids research · 2012Article
- Distinct role of Mediator tail module in regulation of SAGA-dependent, TATA-containing genes in yeast.The EMBO journal · 2012Article
- Review
- One small step for Mot1; one giant leap for other Swi2/Snf2 enzymes?Biochimica et biophysica acta · 2011Review
- Domains of Tra1 important for activator recruitment and transcription coactivator functions of SAGA and NuA4 complexes.Molecular and cellular biology · 2011Article
- The SAGA subunit Ada2 functions in transcriptional silencing.Molecular and cellular biology · 2009Article
- The dynamic personality of TATA-binding protein.Trends in biochemical sciences · 2009Article
- A proteomics analysis of yeast Mot1p protein-protein associations: insights into mechanism.Molecular & cellular proteomics : MCP · 2008Article
- Site-specific cross-linking of TBP in vivo and in vitro reveals a direct functional interaction with the SAGA subunit Spt3.Genes & development · 2008Article
- Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome.Genes & development · 2008Article
- Article
- Article
- Regulation of rRNA synthesis by TATA-binding protein-associated factor Mot1.Molecular and cellular biology · 2007Article
- Snf2/Swi2-related ATPase Mot1 drives displacement of TATA-binding protein by gripping DNA.The EMBO journal · 2006Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Spt3 of Saccharomyces cerevisiae is a factor required for normal transcription from particular RNA polymerase II-dependent promoters. Previous genetic and biochemical analyses have shown that Spt3 interacts with the yeast TATA-binding protein (TBP). To identify other factors that might interact with Spt3, we have screened for mutations that, in combination with an spt3 null mutation, lead to inviability. In this way, we have identified a mutation in MOT1, which encodes an ATP-dependent inhibitor of TBP binding to TATA boxes: Previous analyses suggested that Mot1 causes repression in vivo. However, our analysis of mot1 mutants shows that, similar to spt3 mutants, they have decreased levels of transcription from certain genes, suggesting that Mot1 may function as an activator in vivo. In addition, mot1 mutants have other phenotypes in common with spt3 delta mutants, including suppression of the insertion mutation his4-912 delta. Motivated by these Spt3-Mot1 genetic interactions, we tested for genetic interactions between Spt3 and the general transcription factor TFIIA. TFIIA has been shown previously to be functionally related to Mot1. We found that overexpression of TFIIA partially suppresses an spt3 delta mutation, that toa1 mutants have Spt-phenotypes, and that spt3 delta toa1 double mutants are inviable. We believe that, taken together, these data suggest that Spt3, Mot1, and TFIIA cooperate to regulate TBP-DNA interactions, perhaps at the level of TATA box selection in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.