ArticleMolecular and cellular biology1997
Influence of DNA sequence identity on efficiency of targeted gene replacement.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 57 citations in OpenAlex.
- Generation of Leishmania hybrids by whole genomic DNA transformation.PLoS neglected tropical diseases · 2012Article
- PCRless library mutagenesis via oligonucleotide recombination in yeast.Protein science : a publication of the Protein Society · 2010Article
- Regulation of homologous recombination in eukaryotes.Annual review of genetics · 2010Review
- Characterization of both polyunsaturated fatty acid biosynthetic pathways in Schizochytrium sp.Lipids · 2009Article
- RAD59 is required for efficient repair of simultaneous double-strand breaks resulting in translocations in Saccharomyces cerevisiae.DNA repair · 2008Article
- Capture of linear fragments at a double-strand break in yeast.Nucleic acids research · 2007Article
- MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens.Nucleic acids research · 2006Article
- Parameters determining the efficiency of gene targeting in the moss Physcomitrella patens.Nucleic acids research · 2005Article
- Gene targeting in yeast is initiated by two independent strand invasions.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Gene conversion tracts in Saccharomyces cerevisiae can be extremely short and highly directional.Nucleic acids research · 2003Article
- Multiple pathways promote short-sequence recombination in Saccharomyces cerevisiae.Molecular and cellular biology · 2002Article
- DNA-dependent protein kinase suppresses double-strand break-induced and spontaneous homologous recombination.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- The structure-specific endonuclease Ercc1-Xpf is required for targeted gene replacement in embryonic stem cells.The EMBO journal · 2001Article
- Break-induced replication: a review and an example in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- Repair of double-strand breaks by homologous recombination in mismatch repair-defective mammalian cells.Molecular and cellular biology · 2001Article
- Novel function of Rad27 (FEN-1) in restricting short-sequence recombination.Molecular and cellular biology · 2001Article
- The mechanism of mammalian gene replacement is consistent with the formation of long regions of heteroduplex DNA associated with two crossing-over events.Molecular and cellular biology · 2001Article
- Article
- Nucleotide excision repair/TFIIH helicases RAD3 and SSL2 inhibit short-sequence recombination and Ty1 retrotransposition by similar mechanisms.Molecular and cellular biology · 2000Article
- Regulation of mitotic homeologous recombination in yeast. Functions of mismatch repair and nucleotide excision repair genes.Genetics · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a system for analyzing recombination between a DNA fragment released in the nucleus from a single-copy plasmid and a genomic target in order to determine the influence of DNA sequence mismatches on the frequency of gene replacement in Saccharomyces cerevisiae. Mismatching was shown to be a potent barrier to efficient gene replacement, but its effect was considerably ameliorated by the presence of DNA sequences that are identical to the genomic target at one end of a chimeric DNA fragment. Disruption of the mismatch repair gene MSH2 greatly reduces but does not eliminate the barrier to recombination between mismatched DNA fragment and genomic target sequences, indicating that the inhibition of gene replacement with mismatched sequences is at least partially under the control of mismatch repair. We also found that mismatched sequences inhibited recombination between a DNA fragment and the genome only when they were close to the edge of the fragment. Together these data indicate that while mismatches can destabilize the relationship between a DNA fragment and a genomic target sequence, they will only do so if they are likely to be in the heteroduplex formed between the recombining molecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.