ArticleMolecular and cellular biology1997
Repair of site-specific double-strand breaks in a mammalian chromosome by homologous and illegitimate recombination.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
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Who cites it
114 citing papers in PubMed, 273 citations in OpenAlex.
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- A homology independent sequence replacement strategy in human cells using a CRISPR nuclease.Open biology · 2021Article
- A Mechanism to Minimize Errors during Non-homologous End Joining.Molecular cell · 2020Article
- Efficient and modular CRISPR-Cas9 vector system forPlant direct · 2019Article
- Unlocking HDR-mediated nucleotide editing by identifying high-efficiency target sites using machine learning.Scientific reports · 2019Article
- DNA Repair Deficiency in Breast Cancer: Opportunities for Immunotherapy.Journal of oncology · 2019Review
- Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9.BMC biotechnology · 2018Article
- Stem cell-derived clade F AAVs mediate high-efficiency homologous recombination-based genome editing.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- High-resolution mapping of the pericentromeric region on wheat chromosome arm 5AS harbouring the Fusarium head blight resistance QTL Qfhs.ifa-5A.Plant biotechnology journal · 2018Article
- Homology-Directed Repair of a DefectiveFrontiers in plant science · 2018Article
- Breakpoint mapping and haplotype analysis of translocation t(1;12)(q43;q21.1) in two apparently independent families with vascular phenotypes.Molecular genetics & genomic medicine · 2018Article
- Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation.Neuropeptides · 2017Review
- Article
54 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In mammalian cells, chromosomal double-strand breaks are efficiently repaired, yet little is known about the relative contributions of homologous recombination and illegitimate recombination in the repair process. In this study, we used a loss-of-function assay to assess the repair of double-strand breaks by homologous and illegitimate recombination. We have used a hamster cell line engineered by gene targeting to contain a tandem duplication of the native adenine phosphoribosyltransferase (APRT) gene with an I-SceI recognition site in the otherwise wild-type APRT+ copy of the gene. Site-specific double-strand breaks were induced by intracellular expression of I-SceI, a rare-cutting endonuclease from the yeast Saccharomyces cerevisiae. I-SceI cleavage stimulated homologous recombination about 100-fold; however, illegitimate recombination was stimulated more than 1,000-fold. These results suggest that illegitimate recombination is an important competing pathway with homologous recombination for chromosomal double-strand break repair in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.