ArticleMolecular and cellular biology1997
The mammalian homolog of yeast Sec13p is enriched in the intermediate compartment and is essential for protein transport from the endoplasmic reticulum to the Golgi apparatus.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 121 citations in OpenAlex.
- Proteomic and Ubiquitinated Proteome Insights Into ER Stress Responses in Chinese Hamster Ovary Cells Under Mild Hypothermic Conditions.Biotechnology and bioengineering · 2026Article
- Organizing principles underlying COPII-mediated transport.Current opinion in cell biology · 2025Review
- A Tiny Viral Protein, SARS-CoV-2-ORF7b: Functional Molecular Mechanisms.Biomolecules · 2024Article
- Obesity Modifies the Proteomic Profile of the Periodontal Ligament.International journal of molecular sciences · 2023Article
- A Global Screen for Assembly State Changes of the Mitotic Proteome by SEC-SWATH-MS.Cell systems · 2020Article
- Identification of Interactions in the NMD Complex Using Proximity-Dependent Biotinylation (BioID).PloS one · 2016Article
- A trapper keeper for TRAPP, its structures and functions.Cellular and molecular life sciences : CMLS · 2012Review
- TRAPPC9 mediates the interaction between p150 and COPII vesicles at the target membrane.PloS one · 2012Article
- Probabilistic density maps to study global endomembrane organization.Nature methods · 2010Article
- High-quality immunofluorescence of cultured cells.Methods in molecular biology (Clifton, N.J.) · 2010Article
- The differential expression of aqueous soluble proteins in breast normal and cancerous tissues in relation to stage and grade of patients.Journal of biomedicine & biotechnology · 2010Article
- Integrity of the early secretory pathway promotes, but is not required for, severe acute respiratory syndrome coronavirus RNA synthesis and virus-induced remodeling of endoplasmic reticulum membranes.Journal of virology · 2010Article
- The yeast Golgi apparatus: insights and mysteries.FEBS letters · 2009Review
- p31 deficiency influences endoplasmic reticulum tubular morphology and cell survival.Molecular and cellular biology · 2009Article
- A novel small molecule regulator of guanine nucleotide exchange activity of the ADP-ribosylation factor and golgi membrane trafficking.The Journal of biological chemistry · 2008Article
- A dynamic view of hepatitis C virus replication complexes.Journal of virology · 2008Article
- Two mammalian Sec16 homologues have nonredundant functions in endoplasmic reticulum (ER) export and transitional ER organization.Molecular biology of the cell · 2007Article
- Sec16 defines endoplasmic reticulum exit sites and is required for secretory cargo export in mammalian cells.Traffic (Copenhagen, Denmark) · 2006Article
- The Ca2+-binding protein ALG-2 is recruited to endoplasmic reticulum exit sites by Sec31A and stabilizes the localization of Sec31A.Molecular biology of the cell · 2006Article
- A locus for familial generalized lentiginosis without systemic involvement maps to chromosome 4q21.1-q22.3.Human genetics · 2005Article
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The role of COPII components in endoplasmic reticulum (ER)-Golgi transport, first identified in the yeast Saccharomyces cerevisiae, has yet to be fully characterized in higher eukaryotes. A human cDNA whose predicted amino acid sequence showed 70% similarity to the yeast Sec13p has previously been cloned. Antibodies raised against the human SEC13 protein (mSEC13) recognized a cellular protein of 35 kDa in both the soluble and membrane fractions. Like the yeast Sec13p, mSEC13 exist in the cytosol in both monomeric and higher-molecular-weight forms. Immunofluorescence microscopy localized mSEC13 to the characteristic spotty ER-Golgi intermediate compartment (ERGIC) in cells of all species examined, where it colocalized well with the KDEL receptor, an ERGIC marker, at 15 degrees C. Immunoelectron microscopy also localized mSEC13 to membrane structures close to the Golgi apparatus. mSEC13 is essential for ER-to-Golgi transport, since both the His6-tagged mSEC13 recombinant protein and the affinity-purified mSEC13 antibody inhibited the transport of restrictive temperature-arrested vesicular stomatitis virus G protein from the ER to the Golgi apparatus in a semi-intact cell assay. Moreover, cytosol immunodepleted of mSEC13 could no longer support ER-Golgi transport. Transport could be restored in a dose-dependent manner by a cytosol fraction enriched in the high-molecular-weight mSEC13 complex but not by a fraction enriched in either monomeric mSEC13 or recombinant mSEC13. As a putative component of the mammalian COPII complex, mSEC13 showed partially overlapping but mostly different properties in terms of localization, membrane recruitment, and dynamics compared to that of beta-COP, a component of the COPI complex.
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