ArticleMolecular and cellular biology1997
An Ssn6-Tup1-dependent negative regulatory element controls sporulation-specific expression of DIT1 and DIT2 in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 51 citations in OpenAlex.
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- Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae.Scientific reports · 2016Article
- The molecular mechanism of sporocyteless/nozzle in controlling Arabidopsis ovule development.Cell research · 2015Article
- Spore formation in plants: sporocyteless and more.Cell research · 2015Article
- Stress and polyamine metabolism in fungi.Frontiers in chemistry · 2013Review
- Sporulation in the budding yeast Saccharomyces cerevisiae.Genetics · 2011Review
- The general transcriptional repressor Tup1 is required for dimorphism and virulence in a fungal plant pathogen.PLoS pathogens · 2011Article
- Avoiding unscheduled transcription in shared promoters: Saccharomyces cerevisiae Sum1p represses the divergent gene pair SPS18-SPS19 through a midsporulation element (MSE).FEMS yeast research · 2009Article
- Genome reprogramming during sporulation.The International journal of developmental biology · 2009Review
- Gene expression from random libraries of yeast promoters.Genetics · 2006Article
- Components of the ESCRT pathway, DFG16, and YGR122w are required for Rim101 to act as a corepressor with Nrg1 at the negative regulatory element of the DIT1 gene of Saccharomyces cerevisiae.Molecular and cellular biology · 2005Article
- RSC1 and RSC2 are required for expression of mid-late sporulation-specific genes in Saccharomyces cerevisiae.Eukaryotic cell · 2004Article
- Functional dissection of the global repressor Tup1 in yeast: dominant role of the C-terminal repression domain.Genetics · 2002Article
- Article
- Roles of transcription factor Mot3 and chromatin in repression of the hypoxic gene ANB1 in yeast.Molecular and cellular biology · 2000Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sporulation of the yeast Saccharomyces cerevisiae is a process of cellular differentiation that occurs in MATa/MAT alpha diploid cells in response to starvation. The sporulation-specific genes DIT1 and DIT2, which are required for spore wall formation, are activated midway through the sporulation program, with maximal transcript accumulation occurring at the time of prospore enclosure. In this study, we have identified a negative regulatory element, termed NREDIT, that is located between the start sites of transcription of these divergently transcribed genes. This element, which prevents expression of the DIT1 and DIT2 genes during vegetative growth, reduces expression of a CYC1-lacZ reporter gene more than 1,000-fold and acts in an orientation- and position-independent manner. We found that the ability of NREDIT to turn of expression of the reporter gene and the chromosomal DIT1 and DIT2 genes in vegetative cells requires the Ssn6-Tup1 repression complex. Interestingly, NREDIT-mediated repression of the reporter gene is maintained during sporulation. Derepression during sporulation requires complex interactions among several cis-acting elements. These are present on an approximately 350-bp DNA fragment extending from NREDIT to the TATA box and an approximately 125-bp fragment spanning the TATA box of DIT1. Additionally, a region of NREDIT which is very similar in sequence to UASSPS4, an element that activates gene expression midway through sporulation, contributes both to vegetative repression and to sporulation-specific induction of DIT1. We propose a model to explain the requirement for multiple elements in overcoming NREDIT-mediated repression during sporulation.
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