ArticleJournal of virology1996
Brome mosaic virus helicase- and polymerase-like proteins colocalize on the endoplasmic reticulum at sites of viral RNA synthesis.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
125 citing papers in PubMed, 209 citations in OpenAlex.
- Exogenous and endogenous dsRNAs perceived by plant Dicer-like 4 protein in the RNAi-depleted cellular context.Cellular & molecular biology letters · 2023Article
- Manipulation of the Cellular Membrane-Cytoskeleton Network for RNA Virus Replication and Movement in Plants.Viruses · 2023Review
- A conserved viral amphipathic helix governs the replication site-specific membrane association.PLoS pathogens · 2022Article
- Ultrastructural Features of Membranous Replication Organelles Induced by Positive-Stranded RNA Viruses.Cells · 2021Review
- Genome organization and interaction with capsid protein in a multipartite RNA virus.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Determination of Protein Interactions among Replication Components of Apple Necrotic Mosaic Virus.Viruses · 2020Article
- The Tug-of-War between Plants and Viruses: Great Progress and Many Remaining Questions.Viruses · 2019Review
- Killing two birds with one stone: How do Plant Viruses Break Down Plant Defenses and Manipulate Cellular Processes to Replicate Themselves?Journal of plant biology = Singmul Hakhoe chi · 2019Review
- Host Lipids in Positive-Strand RNA Virus Genome Replication.Frontiers in microbiology · 2019Review
- The TRPV4 channel links calcium influx to DDX3X activity and viral infectivity.Nature communications · 2018Article
- The Superimposed Deubiquitination Effect of OTULIN and Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Nsp11 Promotes Multiplication of PRRSV.Journal of virology · 2018Article
- Host Pah1p phosphatidate phosphatase limits viral replication by regulating phospholipid synthesis.PLoS pathogens · 2018Article
- Cowpea chlorotic mottle bromovirus replication proteins support template-selective RNA replication in Saccharomyces cerevisiae.PloS one · 2018Article
- Three-Dimensional Analysis of Chloroplast Structures Associated with Virus Infection.Plant physiology · 2018Article
- Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Plant Virus Replication.Frontiers in plant science · 2018Review
- Nonstructural protein 5B promotes degradation of the NORE1A tumor suppressor to facilitate hepatitis C virus replication.Hepatology (Baltimore, Md.) · 2017Article
- Endoplasmic Reticulum: The Favorite Intracellular Niche for Viral Replication and Assembly.Viruses · 2016Review
- Positive-strand RNA viruses stimulate host phosphatidylcholine synthesis at viral replication sites.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Role of Mitochondrial Membrane Spherules in Flock House Virus Replication.Journal of virology · 2016Article
- Methods for the characterization of stress granules in virus infected cells.Methods (San Diego, Calif.) · 2015Review
65 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The helicase-like 1a and polymerase-like 2a proteins of brome mosaic virus (BMV) are required for viral RNA replication in vivo, are present in membrane-bound viral RNA polymerase extracts, and share conservation with the many other members of the alphavirus-like superfamily. To better understand BMV RNA replication and BMV-host interactions, we used confocal microscopy and double-label immunofluorescence to determine and compare the sites of 1a, 2a, and nascent viral RNA accumulation in BMV-infected barley protoplasts. 1a and 2a showed nearly complete colocalization throughout infection, accumulating in defined cytoplasmic spots usually adjacent to or surrounding the nucleus. These spots grew throughout infection and by 16 h postinoculation often assumed a vesicle-like appearance. The BMV RNA replication complex incorporated 5-bromouridine 5'-triphosphate into RNA in vitro and in vivo, allowing immunofluorescent detection of nascent RNA. The cytoplasmic sites of BMV-specific RNA synthesis coincided with the sites of 1a and 2a accumulation, and at the resolution of confocal microscopy, all sites of 1a and 2a accumulation were sites of BMV RNA synthesis. Double-label immunofluorescence detection of selected subcellular markers and 1a or 2a showed that BMV replication complexes were tightly associated with markers for the endoplasmic reticulum but not the medial Golgi or later compartments of the cellular secretory pathway. Defining this association of BMV RNA replication complexes with endoplasmic reticulum markers should assist in identifying and characterizing host factors involved in BMV RNA replication.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.