ArticleMolecular biology of the cell1996
Differential localization of syntaxin isoforms in polarized Madin-Darby canine kidney cells.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 234 citations in OpenAlex.
- Vamp3/syntaxin 4 mediates the basolateral membrane fusion of TfR transcytosis across the BBB and is exploited by pathogenicProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Syntaxin 3B: A SNARE Protein Required for Vision.International journal of molecular sciences · 2024Review
- Inverted apicobasal polarity in health and disease.Journal of cell science · 2024Review
- Two roads diverged in a cell: insights from differential exosome regulation in polarized cells.Frontiers in cell and developmental biology · 2024Review
- VAMP5 and distinct sets of cognate Q-SNAREs mediate exosome release.Cell structure and function · 2023Article
- A CRISPR screen in intestinal epithelial cells identifies novel factors for polarity and apical transport.eLife · 2023Article
- Role of SNARE Proteins in the Insertion of KCa3.1 in the Plasma Membrane of a Polarized Epithelium.Frontiers in physiology · 2022Article
- PLRP2 selectively localizes synaptic membrane proteins via acyl-chain remodeling of phospholipids.Journal of lipid research · 2020Article
- Effects of syntaxins 2, 3, and 4 on rat and human epithelial sodium channel (ENaC) in Xenopus laevis oocytes.Pflugers Archiv : European journal of physiology · 2020Article
- The cell biology of the retinal pigment epithelium.Progress in retinal and eye research · 2020Review
- Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs.The EMBO journal · 2019Article
- Quantitative proteomics of MDCK cells identify unrecognized roles of clathrin adaptor AP-1 in polarized distribution of surface proteins.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Choroid plexus epithelial cells express the adhesion protein P-cadherin at cell-cell contacts and syntaxin-4 in the luminal membrane domain.American journal of physiology. Cell physiology · 2018Article
- Iron transport kinetics through blood-brain barrier endothelial cells.Biochimica et biophysica acta. General subjects · 2018Article
- Soluble syntaxin 3 functions as a transcriptional regulator.The Journal of biological chemistry · 2018Article
- Tracking Endocytosis and Intracellular Trafficking of Epitope-tagged Syntaxin 3 by Antibody Feeding in Live, Polarized MDCK Cells.Bio-protocol · 2018Article
- Monoubiquitination of syntaxin 3 leads to retrieval from the basolateral plasma membrane and facilitates cargo recruitment to exosomes.Molecular biology of the cell · 2017Article
- Disruption of Rab8a and Rab11a causes formation of basolateral microvilli in neonatal enteropathy.Journal of cell science · 2017Article
- Article
- MAL Is a Regulator of the Recruitment of Myelin Protein PLP to Membrane Microdomains.PloS one · 2016Article
29 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Syntaxins, integral membrane proteins that are part of the ubiquitous membrane fusion machinery, are thought to act as target membrane receptors during the process of vesicle docking and fusion. Several isoforms of the syntaxin family have been previously identified in mammalian cells, some of which are localized to the plasma membrane. We investigated the subcellular localization of these putative plasma membrane syntaxins in polarized epithelial cells, which are characterized by the presence of distinct apical and basolateral plasma membrane domains. Syntaxins 2, 3, and 4 were found to be endogenously present in Madin-Darby canine kidney cells. The localization of syntaxins 1A, 1B, 2, 3, and 4 in stably transfected Madin-Darby canine kidney cell lines was studied with confocal immunofluorescence microscopy. Each syntaxin isoform was found to have a unique pattern of localization. Syntaxins 1A and 1B were present only in intracellular structures, with little or no apparent plasma membrane staining. In contrast, syntaxin 2 was found on both the apical and basolateral surface, whereas the plasma membrane localization of syntaxins 3 and 4 were restricted to the apical or basolateral domains, respectively. Syntaxins are therefore the first known components of the plasma membrane fusion machinery that are differentially localized in polarized cells, suggesting that they may play a central role in targeting specificity.
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