ArticleMolecular biology of the cell1996
NG2 proteoglycan and the actin-binding protein fascin define separate populations of actin-containing filopodia and lamellipodia during cell spreading and migration.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 41 citations in OpenAlex.
- Functional roles of CSPG4/NG2 in chondrosarcoma.International journal of experimental pathology · 2016Article
- Transduction of extracellular cues into cell polarity: the role of the transmembrane proteoglycan NG2.Molecular neurobiology · 2014Review
- NG2 cells: Properties, progeny and origin.Brain research reviews · 2010Review
- A novel function for p53: regulation of growth cone motility through interaction with Rho kinase.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- NG2-expressing cells in the nervous system: role of the proteoglycan in migration and glial-neuron interaction.Journal of anatomy · 2005Review
- The multi-PDZ domain protein MUPP1 is a cytoplasmic ligand for the membrane-spanning proteoglycan NG2.Journal of cellular biochemistry · 2000Article
- Article
- Cytoskeletal reorganization induced by engagement of the NG2 proteoglycan leads to cell spreading and migration.Molecular biology of the cell · 1999Article
- Fibronectin regulates assembly of actin filaments and focal contacts in cultured cells via the heparin-binding site in repeat III13.Molecular biology of the cell · 1999Article
- Fascin, an actin-bundling protein, induces membrane protrusions and increases cell motility of epithelial cells.Molecular biology of the cell · 1998Article
- Characterization of cell-matrix adhesion requirements for the formation of fascin microspikes.Molecular biology of the cell · 1997Article
- A role for the NG2 proteoglycan in glioma progression.Cell adhesion & migrationReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The transmembrane proteoglycan NG2 is able to interact both with components of the extracellular matrix and with the actin cytoskeleton. An examination of the distribution of NG2 during cell spreading suggests that NG2 can associate with two distinct types of actin-containing cytoskeletal structures, depending on the nature of the stimulus derived from the substratum. On fibronectin-coated dishes, cell surface NG2 associates exclusively with stress fibers developing within the cell. On poly-L-lysine-coated dishes, cell surface NG2 is associated with radial processes extending from the cell periphery. Spreading on fibronectin/poly-L-lysine mixtures, as well as on matrix components such as laminin, tenascin, and type VI collagen, produces cells with mosaic characteristics, i.e., NG2 is associated with both types of structures. NG2-positive radial processes are distinct from a second population of radial structures that contain fascin. NG2-positive extensions appear to be individual self-contained units (filopodia), whereas fascin is associated with actin ribs within sheets of membrane (lamellipodia). NG2- and fascin-positive structures are often localized to opposite poles of spreading cells, suggesting a possible role for the two classes of cellular extensions in the establishment of cell polarity during morphogenesis or migration. Time lapse imaging confirms the presence of lamellipodia on the leading edges of migrating cells, while numerous filopodia are present on trailing edges.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.