ArticleMolecular biology of the cell1996
Is outer arm dynein intermediate chain 1 multifunctional?
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 81 citations in OpenAlex.
- Consensus nomenclature for dyneins and associated assembly factors.The Journal of cell biology · 2022Article
- Sperm Redox System Equilibrium: Implications for Fertilization and Male Fertility.Advances in experimental medicine and biology · 2022Article
- Review
- Genome-Wide Identification and Characterization of WD40 Protein Genes in the Silkworm, Bombyx mori.International journal of molecular sciences · 2018Article
- General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase.Molecular biology of the cell · 2017Article
- Axonemal Dynein Arms.Cold Spring Harbor perspectives in biology · 2016Review
- Sperm dysfunction and ciliopathy.Reproductive medicine and biology · 2016Review
- Circovirus transport proceeds via direct interaction of the cytoplasmic dynein IC1 subunit with the viral capsid protein.Journal of virology · 2015Article
- Article
- Chlamydomonas ODA10 is a conserved axonemal protein that plays a unique role in outer dynein arm assembly.Molecular biology of the cell · 2013Article
- Integrated control of axonemal dynein AAA(+) motors.Journal of structural biology · 2012Review
- Tubulin-dynein system in flagellar and ciliary movement.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2012Review
- A unified taxonomy for ciliary dyneins.Cytoskeleton (Hoboken, N.J.) · 2011Article
- Article
- Nme protein family evolutionary history, a vertebrate perspective.BMC evolutionary biology · 2009Article
- The mammalian Nm23/NDPK family: from metastasis control to cilia movement.Molecular and cellular biochemistry · 2009Review
- A common variant in combination with a nonsense mutation in a member of the thioredoxin family causes primary ciliary dyskinesia.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Analysis of cytoskeletal and motility proteins in the sea urchin genome assembly.Developmental biology · 2006Article
- Modulation of Chlamydomonas reinhardtii flagellar motility by redox poise.The Journal of cell biology · 2006Article
- Identification of predicted human outer dynein arm genes: candidates for primary ciliary dyskinesia genes.Journal of medical genetics · 2006Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The outer arm dynein of sea urchin sperm axoneme contains three intermediate chains (IC1, IC2, and IC3; M(r) 128,000, 98,000, and 74,000, respectively). IC2 and IC3 are members of the WD family; the WD motif is responsible for a protein-protein interaction. We describe here the molecular cloning of IC1. IC1 has a unique primary structure, the N-terminal part is homologous to the sequence of thioredoxin, the middle part consists of three repetitive sequences homologous to the sequence of nucleoside diphosphate kinase, and the C-terminal part contains a high proportion of negatively charged glutamic acid residues. Thus, IC1 is a novel dynein intermediate chain distinct from IC2 and IC3 and may be a multifunctional protein. The thioredoxin-related part of IC1 is more closely related to those of two redox-active Chlamydomonas light chains than thioredoxin. Antibodies were prepared against the N-terminal and middle domains of IC1 expressed as His-tagged proteins in bacteria. These antibodies cross-reacted with some dynein polypeptides (potential homologues of IC1) from distantly related species. We propose here that the three intermediate chains are the basic core units of sperm outer arm dynein because of their ubiquitous existence. The recombinant thioredoxin-related part of IC1 and outer arm dyneins from sea urchin and distantly related species were specifically bound to and eluted from a phenylarsine oxide affinity column with 2-mercaptoethanol, indicating that they contain vicinal dithiols competent to undergo reversible oxidation/reduction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.