ArticleMolecular biology of the cell1996
Disruption of actin filaments increases the activity of sodium-conducting channels in human myeloid leukemia cells.
Article in Molecular biology of the cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- Lecithin Prevents Cortical Cytoskeleton Reorganization in Rat Soleus Muscle Fibers under Short-Term Gravitational Disuse.PloS one · 2016Article
- Mechanical characteristics of mesenchymal stem cells under impact of silica-based nanoparticles.Nanoscale research letters · 2014Article
- Cell mechanosensitivity: mechanical properties and interaction with gravitational field.BioMed research international · 2013Review
- Regulation of sodium channel activity by capping of actin filaments.Molecular biology of the cell · 2003Article
- Mechanosensitive cation channels in human leukaemia cells: calcium permeation and blocking effect.The Journal of physiology · 2002Article
- Expression and electrophysiological function of actin in chick cerebellar neurons.Neurochemical research · 2000Article
- Voltage-dependent K+ channels as targets of osmosensing in guard cellsThe Plant cell · 1998Article
- The actin-severing protein gelsolin modulates calcium channel and NMDA receptor activities and vulnerability to excitotoxicity in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Comparison of cellular effects of starch-coated SPIONs and poly(lactic-co-glycolic acid) matrix nanoparticles on human monocytes.International journal of nanomedicineArticle
- Role of the Scaffold Protein MIM in the Actin-Dependent Regulation of Epithelial Sodium Channels (ENaC).Acta naturaeArticle
- Role of submembranous actin cytoskeleton in regulation of non-voltage-gated sodium channels.Doklady. Biochemistry and biophysicsArticle
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the use of the patch clamp technique, the role of cytoskeleton in the regulation of ion channels in plasma membrane of leukemic K562 cells was examined. Single-channel measurements have indicated that disruption of actin filaments with cytochalasin D (CD) resulted in a considerable increase of the activity of non-voltage-gated sodium-permeable channels of 12 pS unitary conductance. Background activity of these channels was low; open probability (po) did not exceed 0.01-0.02. After CD, po grew at least 10-20 times. Cell-attached and whole-cell recordings showed that activation of sodium channels was elicited within 1-3 min after the addition of 10-20 micrograms/ml CD to the bath extracellular solution or in the presence of 5 micrograms/ml CD in the intracellular pipette solution. Preincubation of K562 cells with CD during 1 h also increased drastically the activity of 12 pS sodium channels. Whole-cell measurements confirmed that CD-activated channels were permeable to monovalent cations (preferentially to Na+ and Li+), but not to bivalent cations (Ca2+, Ba2+). Colchicine (1 microM), which affect microtubules, did not alter background channel activity. Our data indicate that actin filaments organization plays an important role in the regulation of sodium-permeable channels which may participate in providing passive Na+ influx in red blood cells.
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