ArticleJournal of clinical microbiology1997
Rapid and effective method for preparation of fecal specimens for PCR assays.
Article in Journal of clinical microbiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 44 citations in OpenAlex.
- Epidemiology and antimicrobial resistance of toxin-producingHealth science reports · 2024Article
- Evaluation of a New Device for Simplifying and Standardizing Stool Sample Preparation for Viral Molecular Testing with Limited Hands-On Time.Journal of clinical microbiology · 2016Article
- Development of a PCR assay for the detection of Spironucleus muris.Journal of the American Association for Laboratory Animal Science : JAALAS · 2013Article
- Genetic analysis of H1N1 influenza virus from throat swab samples in a microfluidic system for point-of-care diagnostics.Journal of the American Chemical Society · 2011Article
- Flotation-A New Method to Circumvent PCR Inhibitors in the Diagnosis of Lawsonia intracellularis.International journal of microbiology · 2009Article
- Spread of infectious chronic bee paralysis virus by honeybee (Apis mellifera L.) feces.Applied and environmental microbiology · 2007Article
- Molecular fingerprinting of Clostridium difficile isolates: pulsed-field gel electrophoresis versus amplified fragment length polymorphism.Journal of clinical microbiology · 2002Article
- Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens.Journal of clinical microbiology · 2001Article
- Comparison of cultivation and PCR-hybridization for detection of Salmonella in porcine fecal and water samples.Journal of clinical microbiology · 2001Article
- PCR detection of Escherichia coli O157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA.Journal of clinical microbiology · 2000Article
- Application of nucleic acid amplification in clinical microbiology.Molecular biotechnology · 1999Review
- Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers.Applied and environmental microbiology · 1998Article
- Diarrheagenic Escherichia coli.Clinical microbiology reviews · 1998Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a novel method for the preparation of fecal specimens for PCR assays. Approximately 100 mg of solid stool or 200 microliters of liquid fecal sample was thoroughly suspended in 1 ml of water. Fecal debris was removed by low-speed centrifugation (2,800 x g for 2 min). The supernatant was then boiled for 10 min in a water bath and further clarified by high-speed centrifugation (12,000 x g for 5 min). Fifty microliters of the clarified supernatant was then purified by Sepharose CL-6B spin column chromatography, and a portion of the purified supernatant was used for PCR. By this method, stools containing enterotoxigenic Escherichia coli H10407 were amplified by colonization factor antigen I fimbrial gene PCR, with a sensitivity of 100 organisms per reaction. The method was also effective for processing stool specimens for Clostridium difficile toxin A and B gene PCRs. This method is rapid, effective, and simple to perform and will improve the applications of PCR to stool specimens for diagnostic purposes.
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