ArticleBiophysical journal1996
Preparation of giant liposomes in physiological conditions and their characterization under an optical microscope.
Article in Biophysical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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Who cites it
113 citing papers in PubMed, 404 citations in OpenAlex.
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- Triacylglycerol-droplet-induced bilayer spontaneous curvature in giant unilamellar vesicles.Biophysical journal · 2024Article
- Artificial cells for in vivo biomedical applications through red blood cell biomimicry.Nature communications · 2024Review
- An Efficient Method for the Production of High-Purity Bioinspired Large Unilamellar Vesicles.ACS synthetic biology · 2024Article
- Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes.International journal of molecular sciences · 2023Review
- Damage Effect of Amorphous Carbon Black Nanoparticle Aggregates on Model Phospholipid Membranes: Surface Charge, Exposure Concentration and Time Dependence.International journal of environmental research and public health · 2023Article
- Assessing membrane material properties from the response of giant unilamellar vesicles to electric fields.Advances in physics: X · 2023Article
- Bending Rigidity, Capacitance, and Shear Viscosity of Giant Vesicle Membranes Prepared by Spontaneous Swelling, Electroformation, Gel-Assisted, and Phase Transfer Methods: A Comparative Study.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Rapid Encapsulation of Reconstituted Cytoskeleton Inside Giant Unilamellar Vesicles.Journal of visualized experiments : JoVE · 2021Article
- Article
- Membrane anchoring facilitates colocalization of enzymes in plant cytochrome P450 redox systems.Communications biology · 2021Article
- Dielectric properties and lamellarity of single liposomes measured by in-liquid scanning dielectric microscopy.Journal of nanobiotechnology · 2021Article
- How to Achieve High Encapsulation Efficiencies for Macromolecular and Sensitive APIs in Liposomes.Pharmaceutics · 2021Article
- Giant Vesicles Produced with Phosphatidylcholines (PCs) and Phosphatidylethanolamines (PEs) by Water-in-Oil Inverted Emulsions.Life (Basel, Switzerland) · 2021Article
- Recurrent dynamics of rupture transitions of giant lipid vesicles at solid surfaces.Biophysical journal · 2021Article
- Bulk Self-Assembly of Giant, Unilamellar Vesicles.ACS nano · 2020Article
- Article
53 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unilamellar liposomes with diameters of 25-100 microns were prepared in various physiological salt solutions, e.g., 100 mM KCl plus 1 mM CaCl2. Successful preparation of the giant liposomes at high ionic strengths required the inclusion of 10-20% of a charged lipid, such as phosphatidylglycerol, phosphatidylserine, phosphatidic acid, or cardiolipin, in phosphatidylcholine or phosphatidylethanolamine. Three criteria were employed to identify unilamellar liposomes, yielding consistent results. Under a phase-contrast microscope those liposomes that showed the thinnest contour and had a vigorously undulating membrane were judged unilamellar. When liposomes were stained with the lipophilic fluorescent dye octadecyl rhodamine B, fluorescence intensities of the membrane of individual liposomes were integer multiples (up to four) of the lowest ones, the least fluorescent liposomes being those also judged unilamellar in the phase-contrast image. Micropipette aspiration test showed that the liposomes judged unilamellar in phase and fluorescence images had an area elastic modulus of approximately 160 dyn/cm, in agreement with literature values. The giant liposomes were stable and retained a concentration gradient of K+ across the membrane, as evidenced in fluorescence images of the K(+)-indicator PBFI encapsulated in the liposomes. Ionophore-induced K+ transport and associated volume change were observed in individual liposomes.
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