ArticleBiophysical chemistry1996
Synthesis and evaluation of Ru-complexes as anisotropy probes for protein hydrodynamics and immunoassays of high-molecular-weight antigens.
Article in Biophysical chemistry, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 69 citations in OpenAlex.
- Is there reversible dimerization of albumin in blood plasma? And does it matter?Experimental physiology · 2024Review
- Controlling the optical and catalytic properties of artificial metalloenzyme photocatalysts using chemogenetic engineering.Chemical science · 2022Article
- Surface-Bound Ruthenium Diimine Organometallic Complexes: Excited-State Properties.Organometallics · 2014Article
- Photophysical studies of bioconjugated ruthenium metal-ligand complexes incorporated in phospholipid membrane bilayers.Inorganic chemistry · 2013Article
- Dynamics of bacteriophage R17 probed with a long-lifetime Ru(II) metal-ligand complex.Journal of fluorescence · 2010Article
- Tuning photophysical properties with ancillary ligands in Ru(II) mono-diimine complexes.Journal of organometallic chemistry · 2009Article
- Interactions between redox complexes and semiconductor quantum dots coupled via a peptide bridge.Journal of the American Chemical Society · 2008Article
- A high throughput fluorescence polarization assay for inhibitors of the GoLoco motif/G-alpha interaction.Combinatorial chemistry & high throughput screening · 2008Article
- Effects of metallic silver particles on the emission properties of [Ru(bpy)(3)].Chemical physics letters · 2003Article
- High-molecular-weight protein hydrodynamics studied with a long-lifetime metal-ligand complex.Biochimica et biophysica acta · 2002Article
- Enhanced Emission Induced by FRET from a Long-Lifetime, Low Quantum Yield Donor to a Long-Wavelength, High Quantum Yield Acceptor.Journal of fluorescence · 2002Article
- Lateral diffusion coefficients in membranes measured by resonance energy transfer and a new algorithm for diffusion in two dimensions.Biophysical journal · 2002Article
- Long-wavelength long-lifetime luminophores.Analytical chemistry · 2001Article
- Albumin-bound MRI contrast agents: the dilemma of the rotational correlation time.Magma (New York, N.Y.) · 2001Article
- On the possibility of long-wavelength long-lifetime high-quantum-yield luminophores.Analytical biochemistry · 2001Article
- Long-lifetime lipid rhenium metal-ligand complex for probing membrane dynamics on the microsecond timescale.Chemistry and physics of lipids · 1999Article
- Polarization sensing with visual detection.Analytical chemistry · 1999Article
- ADVANCES IN FLUORESCENCE SPECTROSCOPY: MULTI-PHOTON EXCITATION, ENGINEERED PROTEINS, MODULATION SENSING AND MICROSECOND RHENIUM METAL-LIGAND COMPLEXES.Acta physica Polonica: A · 1999Article
- Temporally and spectrally resolved imaging microscopy of lanthanide chelates.Biophysical journal · 1998Article
- Synthesis and spectral characterization of a thiol-reactive long-lifetime Ru(II) complex.Analytical biochemistry · 1997Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We investigated three unsymmetrical Ru-complexes, namely [Ru(bpy)2 (phen-ITC)]2+, [Ru(bpy)2(dcbpy)] and [Ru(bpy)2(mcbpy)]+ for use as probes for rotational diffusion and in immunoassays of high-molecular-weight antigens. For this purpose we synthesized reactive forms of these metal-ligand complexes and conjugated them to human serum albumin (HSA). The maximal anisotropies (r0) for the HSA-bound forms in frozen solution are 0.23, 0.17 and 0.14 for the (dcbpy), (mcbpy) and (phen-ITC) derivatives, respectively. The activated Ru metal-ligand complexes have either one or two NHS-esters or an isothiocyanate group as the reactive moiety. The usefulness of these complexes in immunoassays was determined by titration of the labeled HSA with polyclonal anti-HSA. The highest steady state anisotropy (r) values (0.190) were observed for the [Ru(bpy)2(dcbpy)]-labeled HSA on titration with polyclonal antibody. However, a relative increase in the steady state anisotropy (r/r0) on titration with polyclonal antibody was found for the phen-ITC probe (96%), as compared to the dcbpy (83%) or mcbpy (79%) derivatives. These findings were confirmed by time-resolved frequency-domain measurements. In particular the higher mean correlation times calculated for the phen-ITC derivative suggests reduced local probe motion for this probe when bound to HSA as compared to the (mcbpy) and (dcbpy) conjugates.
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