ArticleProceedings of the National Academy of Sciences of the United States of America1996
In vitro and in vivo differentiation into B cells, T cells, and myeloid cells of primitive yolk sac hematopoietic precursor cells expanded > 100-fold by coculture with a clonal yolk sac endothelial cell line.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A niche-like culture system allowing the maintenance of primary human acute myeloid leukemia-initiating cells: a new tool to decipher their chemoresistance and self-renewal mechanisms.Stem cells translational medicine · 2014Trial
- Microporous Hydroxyapatite-Based Ceramics Alter the Physiology of Endothelial Cells through Physical and Chemical Cues.Journal of functional biomaterials · 2023Article
- Hematopoietic stem cell-independent hematopoiesis and the origins of innate-like B lymphocytes.Development (Cambridge, England) · 2019Review
- Blood on the tracks: hematopoietic stem cell-endothelial cell interactions in homing and engraftment.Journal of molecular medicine (Berlin, Germany) · 2017Review
- Modeling murine yolk sac hematopoiesis with embryonic stem cell culture systems.Frontiers in biology · 2014Article
- Differential regulation of CXCL5 by FGF2 in osteoblastic and endothelial niche cells supports hematopoietic stem cell migration.Stem cells and development · 2012Article
- Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
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- Glucocorticoid and growth factor synergism requirement for Notch4 chromatin domain activation.Molecular and cellular biology · 2007Article
- An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis.The Journal of cell biology · 2006Article
- Role of hematopoietic lineage cells as accessory components in blood vessel formation.Cancer science · 2006Review
- Bone marrow dysfunction in mice lacking the cytokine receptor gp130 in endothelial cells.Blood · 2005Article
- Role of hematopoietic stem cells in angiogenesis.International journal of hematology · 2001Review
- Hematopoietic colony-forming cells derived from human embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- In vivo repopulating hematopoietic stem cells are present in the murine yolk sac at day 9.0 postcoitus.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
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3 authors.
Funding
Abstract
The yolk sac, first site of hematopoiesis during mammalian development, contains not only hematopoietic stem cells but also the earliest precursors of endothelial cells. We have previously shown that a nonadherent yolk sac cell population (WGA+, density < 1.077, AA4.1+) can give rise to B cells, T cells and myeloid cells both in vitro and in vivo. We now report on the ability of a yolk sac-derived cloned endothelial cell line (C166) to provide a suitable microenvironment for expansion of these early precursor cells. Single day 10 embryonic mouse yolk sac hematopoietic stem cells wer expanded > 100 fold within 8 days by coculture with irradiated C166 cells. Colony-forming ability was retained for at least three passages in vitro, with retention of the ability to differentiate into T-cell, B-cell, and myeloid lineages. Stem cell properties were maintained by a significant fraction of nonadherent cells in the third passage, although these stem cells expressed a somewhat more mature cell surface phenotype than the initial yolk sac stem cells. When reintroduced into adult allogeneic immunocompromised (scid) hosts, they were able to give rise to all of the leukocyte lineages, including T cells, B cells, and myeloid cells. We conclude that yolk sac endothelial cells can support the stable proliferation of multipotential hematopoietic stem cells, thus generating adequate numbers of cells for study of the mechanisms involved in their subsequent development and differentiation, for in vivo hematopoietic restitution, and for potential use as a vehicle for gene transfer.
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