ArticleProceedings of the National Academy of Sciences of the United States of America1996
A fusion pore phenotype in mast cells of the ruby-eye mouse.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Fusion Pores as Regulators of Quantal Size and Cellular Physiology.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Syntaxin 3, but not syntaxin 4, is required for mast cell-regulated exocytosis, where it plays a primary role mediating compound exocytosis.The Journal of biological chemistry · 2019Article
- Munc18-2, but not Munc18-1 or Munc18-3, controls compound and single-vesicle-regulated exocytosis in mast cells.The Journal of biological chemistry · 2018Article
- Munc13 proteins control regulated exocytosis in mast cells.The Journal of biological chemistry · 2018Article
- Expanding neurochemical investigations with multi-modal recording: simultaneous fast-scan cyclic voltammetry, iontophoresis, and patch clamp measurements.The Analyst · 2016Article
- Vesicular trafficking and signaling for cytokine and chemokine secretion in mast cells.Frontiers in immunology · 2014Review
- Mechanisms of granule membrane recapture following exocytosis in intact mast cells.The Journal of biological chemistry · 2013Article
- Activity-dependent bulk synaptic vesicle endocytosis--a fast, high capacity membrane retrieval mechanism.Molecular neurobiology · 2009Review
- Initial size and dynamics of viral fusion pores are a function of the fusion protein mediating membrane fusion.Biology of the cell · 2008Article
- Disorders of lysosome-related organelle biogenesis: clinical and molecular genetics.Annual review of genomics and human genetics · 2008Review
- Resolution of patch capacitance recordings and of fusion pore conductances in small vesicles.Biophysical journal · 2000Article
- Capacitance flickers and pseudoflickers of small granules, measured in the cell-attached configuration.Biophysical journal · 1998Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Using patch-clamp capacitance and amperometric techniques, we have identified an exocytotic phenotype that affects the function of the fusion pore, the molecular structure that connects the lumen of a secretory vesicle with the extracellular environment during exocytosis. Direct observation of individual exocytotic events in mast cells from the ruby-eye mouse (ru/ru) showed a 3-fold increase in the fraction and duration of transient fusion events with respect to wild-type mice. The fraction of the total fusion events that were transient increased from 0.22 +/- 0.02 (wild type) to 0.65 +/- 0.02 (ru/ru), and the average duration of these events increased from 418 +/- 32 ms (wild type) to 1207 +/- 89 ms (ru/ru). We also show that this phenotype can reduce and delay an evoked secretory response by causing the fusion of vesicles that have been previously emptied by repeated cycles of transient fusion. The exocytotic phenotype that we describe here may be a cause of diseases like platelet storage pool deficiency and prolonged bleeding times for which the ruby-eye mouse serves as an animal model. Furthermore, the identification of the gene causing the fusion pore phenotype reported here will illuminate the molecular mechanisms regulating exocytotic fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.