ArticleInternational journal of legal medicine1996
Effect of environmental factors on PCR-DNA analysis from dental pulp.
Article in International journal of legal medicine, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Review
- Exploring the Correlation between Selected Oral Commensals and Deoxyribonucleic Acid Degradation in Pulp Tissue Extracted from Primary Teeth Stored in Dry and Wet Conditions: AnInternational journal of clinical pediatric dentistry · 2025Article
- Review
- Efficacy of reduced-size short tandem repeat PCR analysis for degraded DNA samples.Genes & genomics · 2021Article
- DNA degradation in human teeth exposed to thermal stress.Scientific reports · 2021Article
- Human identification through DNA analysis of teeth using powder-free method - A case study.The Journal of forensic odonto-stomatology · 2021Article
- Comparative forensic analysis of reverse root canal filing and conventional method for DNA isolation from extracted teeth under different environmental conditions: A prospective study.The Journal of forensic odonto-stomatology · 2020Article
- Ancient dental pulp: Masterpiece tissue for paleomicrobiology.Molecular genetics & genomic medicine · 2020Review
- DNA analysis of the last Brazilian unknown soldier's remains buried in Pistoia (Italy).Forensic sciences research · 2020Article
- The Two Brothers: an enlightening study of ancient Egyptian teeth.British dental journal · 2019Article
- Improved Y-STR typing for disaster victim identification, missing persons investigations, and historical human skeletal remains.International journal of legal medicine · 2018Article
- Usefulness of telomere length in DNA from human teeth for age estimation.International journal of legal medicine · 2018Article
- Dental color measurement to predict DNA concentration in incinerated teeth for human identification.PloS one · 2018Article
- Differential nuclear and mitochondrial DNA preservation in post-mortem teeth with implications for forensic and ancient DNA studies.PloS one · 2015Article
- The tooth for molecular analysis and identification : a forensic approach.The Journal of forensic odonto-stomatology · 2012Article
- Y-STR analysis of degraded DNA using reduced-size amplicons.International journal of legal medicine · 2007Article
- A new "miniSTR-multiplex" displaying reduced amplicon lengths for the analysis of degraded DNA.International journal of legal medicine · 2006Article
- Forensic DNA analysis of heat-exposed unrestored teeth: Temperature and duration effects.Journal of oral and maxillofacial pathology : JOMFPArticle
- Comparison of Gender Determination from Dental Pulp and Dentin after Exposure to Various Environmental Conditions: A Polymerase Chain Reaction-based SRY Gene Study.Contemporary clinical dentistryArticle
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was designed to observe the results of DNA typing on teeth subjected to aging, different temperatures and various environmental factors. A total of 570 teeth were studied. The study included the analysis of the PCR-based polymorphisms HLA DQA1, D1S80, HUMTH01, HUMFES/FPS and the XY homologous gene amelogenin. In general the best results were obtained with the XY homologous gene amelogenin, followed by the two STRs studied (HUMTH01 and HUMFES/FPS). The small fragment sizes and the method of detection used after PCR amplification are the main factors explaining this fact. In general, teeth submerged in water gave the poorest results. Teeth exposed to outdoor conditions provided better results than teeth buried in sand or soil, but even in these cases good results were obtained. Up to 4 degree C, temperature had only a slight influence on the results. Positive results were obtained in most cases at high temperatures (400 degrees C for 2 min) which are rarely reached in practical casework. Positive typing results for the XY homologous gene amelogenin and the STRs were obtained from teeth 10-30 years old. The usefulness of dental pulp for identification purposes is exemplified in some actual cases.
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