ArticlePharmaceutical research1996
Human dipeptide transporter, hPEPT1, stably transfected into Chinese hamster ovary cells.
Article in Pharmaceutical research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 49 citations in OpenAlex.
- Characterization and design of dipeptide media formulation for scalable therapeutic production.Applied microbiology and biotechnology · 2025Review
- Article
- Pharmacophore-based discovery of ligands for drug transporters.Advanced drug delivery reviews · 2006Review
- In vitro and pharmacophore-based discovery of novel hPEPT1 inhibitors.Pharmaceutical research · 2005Article
- Stable transfection of MDCK cells with epitope-tagged human PepT1.Pharmaceutical research · 2004Article
- Monitoring intracellular pH changes in response to osmotic stress and membrane transport activity using 5-chloromethylfluorescein.AAPS pharmSci · 2002Article
- Intestinal peptide transport systems and oral drug availability.Pharmaceutical research · 1999Review
- Evidence for overlapping substrate specificity between large neutral amino acid (LNAA) and dipeptide (hPEPT1) transporters for PD 158473, an NMDA antagonist.Pharmaceutical research · 1999Article
- Cellular uptake mechanism of amino acid ester prodrugs in Caco-2/hPEPT1 cells overexpressing a human peptide transporter.Pharmaceutical research · 1998Article
- Sequence alignments of the H(+)-dependent oligopeptide transporter family PTR: inferences on structure and function of the intestinal PET1 transporter.Pharmaceutical research · 1997Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
purposeA cDNA encoding the H(+)-coupled peptide transporter, hPEPT1, has previously been cloned from human ileum (8). The objective of this study was to establish a stably transfected cell line expressing hPEPT1 in mammalian cell culture.
methodsThe hPEPT1 cDNA was subcloned into an expression vector carrying the CMV promoter and a neomycin resistance gene. This vector, pCDNA3-PEPT1, was transiently transfected into several cell lines to identify those capable of expressing PEPT1 transport function. CHO cells were selected and stably transfected with PEPT1 (CHO-PEPT1). Dipeptide transport activity was measured with 3H-Gly-Sar, in the presence and absence of inhibitors.
resultsThe clonal cell line, CHO-PEPT1, displayed high transport activity. Dipeptide transport was sensitive to pH and specific for dipeptides and other small peptides. Peptidomimetic antibiotics, such as cephalexin, were competitors for peptide transport.
conclusionsThe stably transfected cell line, CHO-PEPT1 exhibits enhanced transport over that of cell lines with native expression of PEPT1, and therefore, represents a useful tool for rapid screening of drugs that utilize the peptide transporter in the human intestine for absorption.
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