ArticleJournal of bacteriology1996
Amino acid substitutions in membrane-spanning domains of Hol1, a member of the major facilitator superfamily of transporters, confer nonselective cation uptake in Saccharomyces cerevisiae.
Article in Journal of bacteriology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Continuous Directed Evolution of a Short-Lived Plant Histidinol Dehydrogenase.ACS synthetic biology · 2026Article
- Genomic Analysis Reveals Diversified and Stress-Responsive Transport Repertoire inJournal of fungi (Basel, Switzerland) · 2026Article
- Genomic Data Mining Reveals Abundant Uncharacterized Transporters inJournal of fungi (Basel, Switzerland) · 2022Article
- Translational autoregulation of the S. cerevisiae high-affinity polyamine transporter Hol1.Molecular cell · 2021Article
- The mycoparasitic fungusEvolutionary applications · 2018Article
- Yeast Fex1p Is a Constitutively Expressed Fluoride Channel with Functional Asymmetry of Its Two Homologous Domains.The Journal of biological chemistry · 2015Article
- MFS transporters required for multidrug/multixenobiotic (MD/MX) resistance in the model yeast: understanding their physiological function through post-genomic approaches.Frontiers in physiology · 2014Review
- Functional characterization of a eukaryotic melibiose transporter.Plant physiology · 2011Article
- Identification of putative regulatory upstream ORFs in the yeast genome using heuristics and evolutionary conservation.BMC bioinformatics · 2007Article
- Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Amiloride uptake and toxicity in fission yeast are caused by the pyridoxine transporter encoded by bsu1+ (car1+).Eukaryotic cell · 2005Article
- Divalent cation block of inward currents and low-affinity K+ uptake in Saccharomyces cerevisiae.Journal of bacteriology · 1999Article
- Posttranscriptional control of gene expression in yeast.Microbiology and molecular biology reviews : MMBR · 1998Review
- The yeast transcription factor genes YAP1 and YAP2 are subject to differential control at the levels of both translation and mRNA stability.Nucleic acids research · 1998Article
- Trinucleotide insertions, deletions, and point mutations in glucose transporters confer K+ uptake in Saccharomyces cerevisiae.Molecular and cellular biology · 1998Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Selection for the ability of Saccharomyces cerevisiae cells to take up histidinol, the biosynthetic precursor to histidine, results in dominant mutations at HOL1. The DNA sequence of HOL1 was determined, and it predicts a 65-kDa protein related to the major facilitator family (drug resistance subfamily) of putative transport proteins. Two classes of mutations were obtained: (i) those that altered the coding region of HOL1, conferring the ability to take up histidinol; and (ii) cis-acting mutations (selected in a mutant HOL1-1 background) that increased expression of the Hol1 protein. The ability to transport histidinol and other cations was conferred by single amino acid substitutions at any of three sites located within putative membrane-spanning domains of the transporter. These mutations resulted in the conversion of a small hydrophobic amino acid codon to a phenylalanine codon. Selection for spontaneous mutations that increase histidinol uptake by such HOL1 mutants resulted in mutations that abolish the putative start codon of a six-codon open reading frame located approximately 171 nucleotides downstream of the transcription initiation site and 213 nucleotides upstream of the coding region of HOL1. This single small upstream open reading frame (uORF) confers translational repression upon HOL1; genetic disruption of the putative start codon of the uORF results in a 5- to 10-fold increase in steady-state amounts of Hol1 protein without significantly affecting the level of HOL1 mRNA expression.
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