ArticleThe Plant cell1996
Protein targeting and integration signal for the chloroplastic outer envelope membrane.
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Article
- Evolution of protein transport to the chloroplast envelope membranes.Photosynthesis research · 2018Review
- The Direct Involvement of Dark-Induced Tic55 Protein in Chlorophyll Catabolism and Its Indirect Role in the MYB108-NAC Signaling Pathway during Leaf Senescence inInternational journal of molecular sciences · 2018Article
- New insights into the mechanism of chloroplast protein import and its integration with protein quality control, organelle biogenesis and development.Journal of molecular biology · 2015Review
- Targeting and assembly of components of the TOC protein import complex at the chloroplast outer envelope membrane.Frontiers in plant science · 2014Review
- Differential age-dependent import regulation by signal peptides.PLoS biology · 2012Article
- Small heat shock protein Hsp17.8 functions as an AKR2A cofactor in the targeting of chloroplast outer membrane proteins in Arabidopsis.Plant physiology · 2011Article
- Joining forces: the interface of gravitropism and plastid protein import.Plant signaling & behavior · 2009Article
- Tic21 is an essential translocon component for protein translocation across the chloroplast inner envelope membrane.The Plant cell · 2006Article
- Import pathways of chloroplast interior proteins and the outer-membrane protein OEP14 converge at Toc75.The Plant cell · 2004Article
- Chloroplast unusual positioning1 is essential for proper chloroplast positioning.The Plant cell · 2003Article
- A phosphate transporter from Medicago truncatula is expressed in the photosynthetic tissues of the plant and located in the chloroplast envelope.The New phytologist · 2003Article
- Lipid composition of outer leaflet of chloroplast outer envelope determines topology of OEP7.Molecular biology of the cell · 2001Article
- Identification of a signal that distinguishes between the chloroplast outer envelope membrane and the endomembrane system in vivo.The Plant cell · 2001Article
- Tomato allene oxide synthase and fatty acid hydroperoxide lyase, two cytochrome P450s involved in oxylipin metabolism, are targeted to different membranes of chloroplast envelope.Plant physiology · 2001Article
- Insertion of OEP14 into the outer envelope membrane is mediated by proteinaceous components of chloroplasts.The Plant cell · 2000Article
- Protein import and routing systems of chloroplasts.The Plant cell · 1999Review
- The role of lipids in plastid protein transport.Plant molecular biology · 1998Review
- Protein translocation into and across the chloroplastic envelope membranes.Plant molecular biology · 1998Review
- Positive charges determine the topology and functionality of the transmembrane domain in the chloroplastic outer envelope protein Toc34.The Journal of cell biology · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most proteins in chloroplasts are encoded by the nuclear genome and synthesized in the cytosol. With the exception of most quter envelope membrane proteins, nuclear-encoded chloroplastic proteins are synthesized with N-terminal extensions that contain the chloroplast targeting information of these proteins. Most outer membrane proteins, however, are synthesized without extensions in the cytosol. Therefore, it is not clear where the chloroplastic outer membrane targeting information resides within these polypeptides. We have analyzed a chloroplastic outer membrane protein, OEP14 (outer envelope membrane protein of 14 kD, previously named OM14), and localized its outer membrane targeting and integration signal to the first 30 amino acids of the protein. This signal consists of a positively charged N-terminal portion followed by a hydrophobic core, bearing resemblance to the signal peptides of proteins targeted to the endoplasmic reticulum. However, a chimeric protein containing this signal fused to a passenger protein did not integrate into the endoplasmic reticulum membrane. Furthermore, membrane topology analysis indicated that the signal inserts into the chloroplastic outer membrane in an orientation opposite to that predicted by the "positive inside" rule.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.