ArticleThe Plant cell1996
An Arabidopsis gene isolated by a novel method for detecting genetic interaction in yeast encodes the GDP dissociation inhibitor of Ara4 GTPase.
Article in The Plant cell, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 50 citations in OpenAlex.
- Plant RABs: Role in Development and in Abiotic and Biotic Stress Responses.Current genomics · 2021Review
- Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection.Molecular & cellular proteomics : MCP · 2015Article
- Conserved and plant-unique mechanisms regulating plant post-Golgi traffic.Frontiers in plant science · 2012Article
- Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering.Molecular & cellular proteomics : MCP · 2010Article
- The regulatory RAB and ARF GTPases for vesicular trafficking.Plant physiology · 2008Review
- Secretion marker proteins and cell-wall polysaccharides move through different secretory pathways.Planta · 2007Article
- Analysis of the small GTPase gene superfamily of Arabidopsis.Plant physiology · 2003Article
- Different mechanisms of four aluminum (Al)-resistant transgenes for Al toxicity in Arabidopsis.Plant physiology · 2001Article
- Ara6, a plant-unique novel type Rab GTPase, functions in the endocytic pathway of Arabidopsis thaliana.The EMBO journal · 2001Article
- The Arabidopsis thaliana RER1 gene family: its potential role in the endoplasmic reticulum localization of membrane proteins.Plant molecular biology · 1999Article
- The molecular characterization of transport vesicles.Plant molecular biology · 1998Review
- A distinct cyclin-dependent kinase-activating kinase of Arabidopsis thaliana.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis Ara proteins belong to the Rab/Ypt family of small GTPases, which are implicated in intracellular vesicular traffic. To understand their specific roles in the cell, it is imperative to identify molecules that regulate the GTPase cycle. Such molecules have been found and characterized in animals and yeasts but not in plants. Using a yeast system, we developed a novel method of functional screening to detect interactions between foreign genes and identified this Rab regulator in plants. We found that the expression of the ARA4 gene in yeast ypt mutants causes exaggeration of the mutant phenotype. By introducing an Arabidopsis cDNA library into the ypt1 mutant, we isolated a clone whose coexpression overcame the deleterious effect of ARA4. This gene encodes an Arabidopsis homolog of the Rab GDP dissociation inhibitor (GDI) and was named AtGDI1. The expression of AtGDI1 complemented the yeast sec19-1 (gdi1) mutation. AtGDI1 is expressed almost ubiquitously in Arabidopsis tissues. The method described here indicates the physiological interaction of two plant molecules, Ara4 and GDI, in yeast and should be applicable to other foreign genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.