ArticleThe Journal of physiology1996
Membrane currents and the resting membrane potential in cultured bovine pulmonary artery endothelial cells.
Article in The Journal of physiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 110 citations in OpenAlex.
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- The ion transport, GPCR, and RTK toolkit expression in the human cerebrovascular endothelial cell line, hCMEC/D3: an Omics perspective.Frontiers in physiology · 2025Article
- Activation of Endothelial Large Conductance Potassium Channels Protects against TNF-α-Induced Inflammation.International journal of molecular sciences · 2023Article
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- The capillary Kir channel as sensor and amplifier of neuronal signals: Modeling insights on KProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Self-Adaptation Ability of Zinc Oxide Nanoparticles Enables Reliable Cancer Treatments.Nanomaterials (Basel, Switzerland) · 2020Article
- Endothelial Transient Receptor Potential Channels and Vascular Remodeling: Extracellular CaFrontiers in physiology · 2019Review
- Fluid Osmolarity Acutely and Differentially Modulates Lymphatic Vessels Intrinsic Contractions and Lymph Flow.Frontiers in physiology · 2018Article
- Kir2.1 and K2P1 channels reconstitute two levels of resting membrane potential in cardiomyocytes.The Journal of physiology · 2017Article
- Biophysics and Physiology of the Volume-Regulated Anion Channel (VRAC)/Volume-Sensitive Outwardly Rectifying Anion Channel (VSOR).Pflugers Archiv : European journal of physiology · 2016Review
- The role of inflammation in hypoxic pulmonary hypertension: from cellular mechanisms to clinical phenotypes.American journal of physiology. Lung cellular and molecular physiology · 2015Review
- Conducted vasoreactivity: the dynamical point of view.Bulletin of mathematical biology · 2015Article
- Electric fields caused by blood flow modulate vascular endothelial electrophysiology and nitric oxide production.Bioelectromagnetics · 2013Article
- Hypoxic pulmonary vasoconstriction.Physiological reviews · 2012Review
- Quantifying mitochondrial and plasma membrane potentials in intact pulmonary arterial endothelial cells based on extracellular disposition of rhodamine dyes.American journal of physiology. Lung cellular and molecular physiology · 2011Article
- Intracellular Na(+) modulates large conductance Ca(2+)-activated K (+) currents in human umbilical vein endothelial cells.Pflugers Archiv : European journal of physiology · 2008Article
- Gabapentin activates ROMK1 channels by a protein kinase A (PKA)-dependent mechanism.British journal of pharmacology · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. We have used the whole-cell patch-clamp technique to characterize the ionic conductances that determine the resting membrane potential in cultured endothelial cells from calf pulmonary artery (CPAE cells). 2. Resting membrane potentials were scattered between -88 and +5 mV with a mean +/- S.E.M. of -26 +/- 3 mV (n = 104). 3. The most prominent membrane current in resting cells was an inwardly rectifying K+ current. This current showed Na(+)-dependent inactivation and was efficiently blocked by external Ba2+ (EC50 = 2.2 microM), but was relatively insensitive to quinine, quinidine and TEA. 4. Hypertonic cell shrinkage inhibited an outwardly rectifying Cl- current, which was also efficiently blocked by 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB; 100 microM), quinine (500 microM) and quinidine (500 microM). 5. A linear, time-independent background current remained after elimination of these two currents. This current was dependent on extracellular monovalent cations with a permeability sequence of Cs+ > Na+ > Li+ >> N-methyl-D-glucamine. It was partially blocked by millimolar concentrations of the divalent cations Ca2+, Ni2+ and Ba2+. Gd3+ (200 microM) had no significant effect on this background current. 6. Continuous measurements of the membrane potential confirm that the three described conductances are the major determinants of the membrane potential. Due to the low slope conductance in the region between -70 and 0 mV, small changes in one of the current components can evoke large depolarizations or hyperpolarizations, which explains the large scattering of the resting membrane potentials.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.