ArticleNucleic acids research1996
Increasing the distance between the snRNA promoter and the 3' box decreases the efficiency of snRNA 3'-end formation.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 19 citations in OpenAlex.
- Article
- TGS1 impacts snRNA 3'-end processing, ameliorates survival motor neuron-dependent neurological phenotypes in vivo and prevents neurodegeneration.Nucleic acids research · 2022Article
- INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.Nature communications · 2020Article
- The Integrator complex controls the termination of transcription at diverse classes of gene targets.Cell research · 2015Article
- Promoter-proximal polyadenylation sites reduce transcription activity.Genes & development · 2012Article
- A subset of Drosophila integrator proteins is essential for efficient U7 snRNA and spliceosomal snRNA 3'-end formation.Molecular and cellular biology · 2011Article
- Progression through the RNA polymerase II CTD cycle.Molecular cell · 2009Review
- The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing.The EMBO journal · 2003Article
- Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA.The EMBO journal · 1998Article
- Alternative 3'-end processing of U5 snRNA by RNase III.Genes & development · 1997Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Chimeric genes which contained the mouse U1b snRNA promoter, portions of the histone H2a or globin coding regions and the U1b 3'-end followed by a histone 3'-end were constructed. The distance between the U1 promoter and the U1 3' box was varied between 146 and 670 nt. The chimeric genes were introduced into CHO cells by stable transfection or into Xenopus oocytes by microinjection. The efficiency of utilization of the U1 3' box, as measured by the relative amounts of transcripts that ended at the U1 3' box and the histone 3'-end, was dependent on the distance between the promoter and 3'-end box. U1 3'-ends were formed with >90% efficiency on transcripts shorter than 200 nt, with 50-70% efficiency on transcripts of 280-400 nt and with only 10-20% efficiency on transcripts >500 nt. Essentially identical results were obtained after stable transfection of CHO cells or after injecting the genes into Xenopus oocytes. The distance between the U1 promoter and the U1 3' box must be <280 nt for efficient transcription termination at the U1 3' box, regardless of the sequence transcribed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.