ArticleThe Journal of cell biology1996
Phosphorylation of linker histone is associated with transcriptional activation in a normally silent nucleus.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 24 citations in OpenAlex.
- Keeping the soma free of transposons: programmed DNA elimination in ciliates.The Journal of biological chemistry · 2011Review
- Article
- The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleus.Molecular biology of the cell · 2006Article
- Spatiotemporal sites of DNA replication in macro- and micronuclei of the ciliate Paramecium caudatum.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Article
- DNA replication and transcription in new macronuclei of Paramecium caudatum exconjugants.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Article
- A nonessential HP1-like protein affects starvation-induced assembly of condensed chromatin and gene expression in macronuclei of Tetrahymena thermophila.Molecular and cellular biology · 1999Article
- An HP1-like protein is missing from transcriptionally silent micronuclei of Tetrahymena.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies have suggested that micronuclear linker histones are phosphorylated by cAMP-dependent protein kinase (PKA) in Tetrahymena (Sweet, M.T., and C.D. Allis. 1993. Chromosoma. 102: 637-647). In this study, we report that a rapid and dramatic phosphorylation of the micronuclear linker histone, delta, occurs early in the sexual pathway, conjugation. Phosphorylated isoforms of delta are detected as early as 30 min after mixing cells of different mating types; blocking pair formation abolishes this induction completely. Phosphorylation of delta is stimulated by the addition of N6-benzoyladenosine 3':5' cyclic monophosphate to starved (nonmating) cells, suggesting that a PKA/cAMP signal transduction pathway is involved. Maximal phosphorylation of delta is observed during meiotic prophase, a period when micronuclei become transcriptionally active. In situ staining, using phospho-delta-specific antibodies combined with [3H]uridine autoradiography, shows that decondensed micronuclear chromatin undergoing active transcription is enriched in phosphorylated delta isoforms. In contrast, condensed inactive micronuclear chromatin is enriched in dephosphorylated delta. These results strongly suggest that phosphorylation of linker histone plays an important and previously unsuspected role in establishing transcriptional competence in micronuclei.
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Registered trials
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