ArticleThe Journal of cell biology1996
Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- Cell cycle control in the early embryonic development of aquatic animal species.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2015Review
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- Quantification of nanoscale density fluctuations by electron microscopy: probing cellular alterations in early carcinogenesis.Physical biology · 2011Article
- The Chinese hamster dihydrofolate reductase replication origin decision point follows activation of transcription and suppresses initiation of replication within transcription units.Molecular and cellular biology · 2006Article
- CpG methylation of DNA restricts prereplication complex assembly in Xenopus egg extracts.Molecular and cellular biology · 2003Article
- Multiple functional elements comprise a Mammalian chromosomal replicator.Molecular and cellular biology · 2003Article
- Multiple sites of replication initiation in the human beta-globin gene locus.Nucleic acids research · 2001Article
- Temporally coordinated assembly and disassembly of replication factories in the absence of DNA synthesis.Nature cell biology · 2000Article
- Long-distance control of origin choice and replication timing in the human beta-globin locus are independent of the locus control region.Molecular and cellular biology · 2000Article
- DNA replication in quiescent cell nuclei: regulation by the nuclear envelope and chromatin structure.Molecular biology of the cell · 1999Article
- Mcm2, but not RPA, is a component of the mammalian early G1-phase prereplication complex.The Journal of cell biology · 1999Article
- Roles of LAP2 proteins in nuclear assembly and DNA replication: truncated LAP2beta proteins alter lamina assembly, envelope formation, nuclear size, and DNA replication efficiency in Xenopus laevis extracts.The Journal of cell biology · 1999Article
- Short DNA fragments without sequence similarity are initiation sites for replication in the chromosome of the yeast Yarrowia lipolytica.Molecular biology of the cell · 1999Article
- Specification of regions of DNA replication initiation during embryogenesis in the 65-kilobase DNApolalpha-dE2F locus of Drosophila melanogaster.Molecular and cellular biology · 1999Article
- Identification of primary initiation sites for DNA replication in the hamster dihydrofolate reductase gene initiation zone.Molecular and cellular biology · 1998Article
- Histone H1 reduces the frequency of initiation in Xenopus egg extract by limiting the assembly of prereplication complexes on sperm chromatin.Molecular biology of the cell · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xenopus egg extracts initiate DNA replication specifically at the dihydrofolate reductase (DHFR) origin locus with intact nuclei from late G1-phase CHO cells as a substrate, but at nonspecific sites when purified DNA is assembled by the extract into an embryonic nuclear structure. Here we show that late G1-phase CHO nuclei can be cycled through an in vitro Xenopus egg mitosis, resulting in the assembly of an embryonic nuclear envelope around G1-phase chromatin. Surprisingly, replication within these chimeric nuclei initiated at a novel specific site in the 5' region of the DHFR structural gene that does not function as an origin in cultured CHO cells. Preferential initiation at this unusual site required topoisomerase II-mediated chromosome condensation during mitosis. Nuclear envelope breakdown and reassembly in the absence of chromosome condensation resulted in nonspecific initiation. Introduction of condensed chromosomes from metaphase-arrested CHO cells directly into Xenopus egg extracts was sufficient to elicit assembly of chimeric nuclei and preferential initiation at this same site. These results demonstrate clearly that chromosome architecture can determine the sites of initiation of replication in Xenopus egg extracts, supporting the hypothesis that patterns of initiation in vertebrate cells are established by higher order features of chromosome structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.