ArticleThe Biochemical journal1996
Activation of mitogen-activated protein kinase couples neurotensin receptor stimulation to induction of the primary response gene Krox-24.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 65 citations in OpenAlex.
- The role of neurotensin as a novel biomarker in the endoscopic screening of high-risk population for developing colorectal neoplasia.Updates in surgery · 2017Trial
- Exploratory Analysis of Plasma Neurotensin as a Novel Biomarker for Early Detection of Colorectal Polyp and Cancer.Hormones & cancer · 2019Article
- Ligand-mediated dephosphorylation signaling for MAP kinase.Cellular signalling · 2018Article
- Neurotensinergic augmentation of glutamate release at the perforant path-granule cell synapse in rat dentate gyrus: Roles of L-Type Ca²⁺ channels, calmodulin and myosin light-chain kinase.Neuropharmacology · 2015Article
- Elucidating the role of neurotensin in the pathophysiology and management of major mental disorders.Behavioral sciences (Basel, Switzerland) · 2014Review
- Diverse roles of neurotensin agonists in the central nervous system.Frontiers in endocrinology · 2013Article
- Neurotensin and its high affinity receptor 1 as a potential pharmacological target in cancer therapy.Frontiers in endocrinology · 2012Article
- Role of protein kinase C and epidermal growth factor receptor signalling in growth stimulation by neurotensin in colon carcinoma cells.BMC cancer · 2011Article
- Insulin-like growth factor-1 receptor transactivation modulates the inflammatory and proliferative responses of neurotensin in human colonic epithelial cells.The Journal of biological chemistry · 2011Article
- Neurotensin stimulates expression of early growth response gene-1 and EGF receptor through MAP kinase activation in human colonic epithelial cells.International journal of cancer · 2007Article
- Neurotensin phosphorylates GSK-3alpha/beta through the activation of PKC in human colon cancer cells.Neoplasia (New York, N.Y.) · 2006Article
- The role of neurotensin in central nervous system pathophysiology: what is the evidence?Journal of psychiatry & neuroscience : JPN · 2006Review
- Involvement of the neurotensin receptor-3 in the neurotensin-induced migration of human microglia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurotensin (NT) is a neuropeptide that is important in a variety of biological processes such as signal transduction and cell growth. NT effects are mediated by a single class of cell-surface receptors, known as neurotensin receptors (NTRs), which exhibit structural features of the G-protein-coupled receptors superfamily. We investigated NTR signalling properties with Chinese hamster ovary (CHO) cells stably transformed with human NTR (hNTR). First, we showed that NTR stimulation by NT induced the activation of the mitogen-activated protein kinases (MAPKs) in time- and dose-dependent manners. Both p42 and p44 MAPK isoforms were retarded in gel-shift assays, which was consistent with their activation by phosphorylation. In addition we showed that NT caused a prolonged activation of MAPK as measured by in-gel kinase assay. Secondly, we demonstrated that NT induced the expression of the growth-related gene Krox-24 at the protein level, as assessed by Western-blot analysis, and at the transcriptional level, as demonstrated in CHO cells transfected with hNTR and a reporter gene for Krox-24. Activation of MAPK and induction of Krox-24 were both prevented by the NTR antagonist SR 48692, confirming the specific action on NTR. Furthermore we observed coupling of NTR to a mitogenic pathway and Krox-24 induction in the human adenocarcinoma cell line HT29, which naturally expresses NTRs. Considering coupling pathways between NTR stimulation and MAPK activation, we observed a partial inhibition by pertussis toxin (PTX) and a complete blockade by the protein kinase C (PKC) inhibitor GF 109203X. Taken together, these results suggest that (1) stimulation of NTR activates the MAPK pathway by mechanisms involving dual coupling to both PTX-sensitive and PTX-insensitive G-proteins as well as PKC activation, and (2) these effects are associated with the induction of Krox-24, which might be a target of MAPK effector.
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