ArticleThe EMBO journal1996
Smooth muscle alternative splicing induced in fibroblasts by heterologous expression of a regulatory gene.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- MBNL1 and PTB cooperate to repress splicing of Tpm1 exon 3.Nucleic acids research · 2013Article
- Understanding the language of Lys36 methylation at histone H3.Nature reviews. Molecular cell biology · 2012Review
- Vascular smooth muscle cell differentiation-2010.Journal of biomedical research · 2010Article
- The biflavonoid isoginkgetin is a general inhibitor of Pre-mRNA splicing.The Journal of biological chemistry · 2008Article
- The transcription-dependent dissociation of P-TEFb-HEXIM1-7SK RNA relies upon formation of hnRNP-7SK RNA complexes.Molecular and cellular biology · 2007Article
- Use of transcriptional synergy to augment sensitivity of a splicing reporter assay.RNA (New York, N.Y.) · 2006Article
- An apparent pseudo-exon acts both as an alternative exon that leads to nonsense-mediated decay and as a zero-length exon.Molecular and cellular biology · 2006Article
- Identification of cells deficient in signaling-induced alternative splicing by use of somatic cell genetics.RNA (New York, N.Y.) · 2002Article
- A double reporter assay for detecting changes in the ratio of spliced and unspliced mRNA in mammalian cells.Nucleic acids research · 2002Article
- A splicing silencer that regulates smooth muscle specific alternative splicing is active in multiple cell types.Nucleic acids research · 2002Article
- Differential alternative splicing activity of isoforms of polypyrimidine tract binding protein (PTB).RNA (New York, N.Y.) · 2001Article
- Polypyrimidine tract binding protein functions as a repressor to regulate alternative splicing of alpha-actinin mutally exclusive exons.Molecular and cellular biology · 1999Article
- Transposable element insertions respecify alternative exon splicing in three Drosophila myosin heavy chain mutants.Genetics · 1998Article
- Role of an inhibitory pyrimidine element and polypyrimidine tract binding protein in repression of a regulated alpha-tropomyosin exon.RNA (New York, N.Y.) · 1998Article
- Splice-junction elements and intronic sequences regulate alternative splicing of the Drosophila myosin heavy chain gene transcript.Genetics · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alternative splicing is a common mechanism for regulating gene expression in different cell types. In order to understand this important process, the trans-acting factors that enforce the choice of particular splicing pathways in different environments must be identified. We have used the rat alpha-tropomyosin gene as a model system of tissue-specific alternative splicing. Exon 3 of alpha-tropomyosin is specifically inhibited in smooth muscle cells allowing the alternative inclusion of exon 2. We have used a novel gene transfer and selection strategy to detect a gene whose expression in fibroblasts is sufficient to switch them to smooth muscle-specific splicing of alpha-tropomyosin and also alpha-actinin. Extracts from the regulating fibroblasts contain an apparently novel 55 kDa protein which binds to RNA elements required for regulation of tropomyosin splicing. This protein is not detected in extracts of non-regulating cells and is therefore a strong candidate cell-specific splicing regulator. These experiments advance our understanding of smooth muscle splicing regulation as well as establishing a means for direct cloning of tissue-specific splicing regulators which have so far been refractory to biochemical analysis.
Indexed as
Identifiers
8947053PMC452453W234632681What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.