ArticleThe EMBO journal1996
Myosin motors with artificial lever arms.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
45 citing papers in PubMed, 146 citations in OpenAlex.
- Article
- Article
- Optical control of fast and processive engineered myosins in vitro and in living cells.Nature chemical biology · 2021Article
- Switch-1 instability at the active site decouples ATP hydrolysis from force generation in myosin II.Cytoskeleton (Hoboken, N.J.) · 2021Article
- Unraveling a Force-Generating Allosteric Pathway of Actomyosin Communication Associated with ADP and PInternational journal of molecular sciences · 2020Article
- Synthetic biology approaches to dissecting linear motor protein function: towards the design and synthesis of artificial autonomous protein walkers.Biophysical reviews · 2020Review
- Phenamacril is a reversible and noncompetitive inhibitor ofThe Journal of biological chemistry · 2019Article
- Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle?International journal of molecular sciences · 2018Review
- Three mammalian tropomyosin isoforms have different regulatory effects on nonmuscle myosin-2B and filamentous β-actinThe Journal of biological chemistry · 2018Article
- The Conserved Lysine-265 Allosterically Modulates Nucleotide- and Actin-binding Site Coupling in Myosin-2.Scientific reports · 2017Article
- Article
- Remote control of myosin and kinesin motors using light-activated gearshifting.Nature nanotechnology · 2014Article
- Article
- Nonmuscle myosin-2: mix and match.Cellular and molecular life sciences : CMLS · 2013Review
- Article
- Kinetic properties and small-molecule inhibition of human myosin-6.FEBS letters · 2012Article
- Engineering controllable bidirectional molecular motors based on myosin.Nature nanotechnology · 2012Article
- Functional characterization of the human myosin-7a motor domain.Cellular and molecular life sciences : CMLS · 2012Article
- Comparative kinetic and functional characterization of the motor domains of human nonmuscle myosin-2C isoforms.The Journal of biological chemistry · 2011Article
- Detailed tuning of structure and intramolecular communication are dispensable for processive motion of myosin VI.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The myosin head consists of a globular catalytic domain and a light chain binding domain (LCBD). The coupling efficiency between ATP hydrolysis and myosin-induced actin movement is known to decline as the LCBD is truncated or destabilized. However, it was not clear whether the observed alteration in the production of force and movement reflects only the mechanical changes to the length of the LCBD or whether these changes also affect the kinetic properties of the catalytic domain. Here we show that replacement of the LCBD with genetically engineered domains of similar rigidity and dimensions produces functional molecular motors with unchanged kinetic properties. The resulting single-chain, single-headed motors were produced in Dictyostelium discoideum and obtained after purification from a standard peptone-based growth medium at levels of up to 12 mg/l. Their actin motility properties are similar or greater than those of native myosin. Rates of 2.5 and 3.3 microm/s were observed for motor domains fused to one or two of these domains, respectively. Their kinetic and functional similarity to the extensively studied myosin subfragment 1 (S1) and their accessibility to molecular genetic approaches makes these simple constructs ideal models for the investigation of chemo-mechanical coupling in the myosin motor.
Indexed as
Identifiers
8947029PMC452428W1514691814What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.