ArticleInfection and immunity1996
Changes in the carboxyl-terminal repeat region affect extracellular activity and glucan products of Streptococcus gordonii glucosyltransferase.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Role of glucosyltransferase R in biofilm interactions between Streptococcus oralis and Candida albicans.The ISME journal · 2020Article
- Codon-optimized fluorescent mTFP and mCherry for microscopic visualization and genetic counterselection of streptococci and enterococci.Journal of microbiological methods · 2015Article
- Glycosyltransferase-mediated Sweet Modification in Oral Streptococci.Journal of dental research · 2015Review
- Streptococcus gordonii glucosyltransferase promotes biofilm interactions with Candida albicans.Journal of oral microbiology · 2014Article
- Regulation of recombination between gtfB/gtfC genes in Streptococcus mutans by recombinase A.TheScientificWorldJournal · 2013Article
- Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms.Caries research · 2011Review
- Streptococcus gordonii's sequenced strain CH1 glucosyltransferase determines persistent but not initial colonization of teeth of rats.Archives of oral biology · 2008Article
- Streptococcus mutans glucan-binding protein-A affects Streptococcus gordonii biofilm architecture.FEMS microbiology letters · 2007Article
- Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria.Microbiology and molecular biology reviews : MMBR · 2006Review
- Inducible antisense RNA expression in the characterization of gene functions in Streptococcus mutans.Infection and immunity · 2005Article
- Effect of an orphan response regulator on Streptococcus mutans sucrose-dependent adherence and cariogenesis.Infection and immunity · 2003Article
- Identification of Streptococcus bovis biotype I strains among S. bovis clinical isolates by PCR.Journal of clinical microbiology · 2002Article
- Genetic analysis of the rgg-gtfG junctional region and its role in Streptococcus gordonii glucosyltransferase activity.Infection and immunity · 2002Article
- Purification, characterization, and molecular analysis of the gene encoding glucosyltransferase from Streptococcus oralis.Infection and immunity · 2000Article
- Ligand-binding properties of the carboxyl-terminal repeat domain of Streptococcus mutans glucan-binding protein A.Journal of bacteriology · 2000Article
- Isolation of an active catalytic core of Streptococcus downei MFe28 GTF-I glucosyltransferase.Journal of bacteriology · 1999Article
- Effect of Leuconostoc mesenteroides NRRL B-512F dextransucrase carboxy-terminal deletions on dextran and oligosaccharide synthesis.Applied and environmental microbiology · 1998Article
- Deletions in the carboxyl-terminal region of Streptococcus gordonii glucosyltransferase affect cell-associated enzyme activity and sucrose-associated accumulation of growing cells.Applied and environmental microbiology · 1997Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Glucans produced by the glucosyltransferase (GTF) of Streptococcus gordonii confer a hard, cohesive phenotype (Spp+) on colonies grown on sucrose agar plates. S. gordonii strains with specific mutations in the region of gtfG that encodes the GTF carboxyl terminus were characterized. In the parental strain Challis CH1, this region included a series of six direct repeats thought to function in glucan binding. The spontaneous mutant strain CH107 had a 585-bp deletion resulting in the loss of three internal direct repeats. Insertional mutagenesis was used to construct strain CH2RPE, which had the parental repeat region but was missing 14 carboxyl-terminal amino acids. The similarly constructed strain CH4RPE had an in-frame addition of 390 nucleotides encoding two additional direct repeats. Although strains CH1, CH2RPE, and CH4RPE all had similar levels of extracellular GTF activity, strain CH107 had less than 15% of the parental activity; however, Western blots (immunoblots) indicated that the amounts of extracellular GTF protein in all four strains were similar. 13C NMR analyses indicated that partially purified GTFs from the Spp+ strains CH1, CH2RPE, and CH4RPE all produced glucans with similar ratios of alpha1,6 and alpha1,3 glucosidic linkages, whereas the Spp- strain CH107 GTF produced primarily alpha1,6-linked glucans. Transformation of strain CH107 with pAMS57, which carries the gtfG positive regulatory determinant, rgg, increased the amount of GTF activity and GTF antibody-reactive protein ca. fivefold but did not confer a hard colony phenotype on sucrose agar plates, suggesting that the type of glucan product affects the sucrose-promoted colony phenotype.
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