ArticleInfection and immunity1996
Candidacidal activity of recombinant human salivary histatin-5 and variants.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 67 citations in OpenAlex.
- Optimizing Histatin 5: Effects of K13 and K17 Substitutions on Proteolytic Stability and Antifungal Activity.Computational and structural biotechnology journal · 2026Article
- Exploring the Healing Powers of Histatins: From Oral Health to Therapeutics.International journal of molecular sciences · 2025Review
- Interaction of a Histidine-Rich Antimicrobial Saliva Peptide with Model Cell Membranes: The Role of Histidines.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Zinc Binding Inhibits Cellular Uptake and Antifungal Activity of Histatin-5 inACS infectious diseases · 2022Article
- Envisaging Antifungal Potential of Histatin 5: A Physiological Salivary Peptide.Journal of fungi (Basel, Switzerland) · 2021Review
- Thrombin-Derived C-Terminal Peptide ReducesAntimicrobial agents and chemotherapy · 2021Article
- Changes of protein levels in human urine reflect the dysregulation of signaling pathways of chronic kidney disease and its complications.Scientific reports · 2020Article
- Structure-Function Insights of Jaburetox and Soyuretox: Novel Intrinsically Disordered Polypeptides Derived from Plant Ureases.Molecules (Basel, Switzerland) · 2020Review
- Spontaneous Formation of Cushioned Model Membranes Promoted by an Intrinsically Disordered Protein.Langmuir : the ACS journal of surfaces and colloids · 2020Article
- Effects of histatin 5 modifications on antifungal activity and kinetics of proteolysis.Protein science : a publication of the Protein Society · 2020Article
- Digital Design of a Universal Rat Intraoral Device for Therapeutic Evaluation of a Topical Formulation againstInfection and immunity · 2019Article
- Specific metallo-protein interactions and antimicrobial activity in Histatin-5, an intrinsically disordered salivary peptide.Scientific reports · 2019Article
- Evaluation of the Antifungal and Wound-Healing Properties of a Novel Peptide-Based Bioadhesive Hydrogel Formulation.Antimicrobial agents and chemotherapy · 2019Article
- Engineering improved variants of the antifungal peptide histatin 5 with reduced susceptibility to Candida albicans secreted aspartic proteases and enhanced antimicrobial potency.The FEBS journal · 2018Article
- Structural Characterization of Histatin 5-Spermidine Conjugates: A Combined Experimental and Theoretical Study.Journal of chemical information and modeling · 2017Article
- Histatin peptides: Pharmacological functions and their applications in dentistry.Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2017Review
- The Antimicrobial Peptides P-113Du and P-113Tri Function against Candida albicans.Antimicrobial agents and chemotherapy · 2016Article
- In vitro properties of designed antimicrobial peptides that exhibit potent antipneumococcal activity and produces synergism in combination with penicillin.Scientific reports · 2015Article
- How does it kill?: understanding the candidacidal mechanism of salivary histatin 5.Eukaryotic cell · 2014Review
- Candidacidal activity of synthetic peptides based on the antimicrobial domain of the neutrophil-derived protein, CAP37.Medical mycology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Human salivary histatins possess fungicidal and bactericidal activities. The current investigation evaluates the structure-function relationship of histatins with regard to their candidacidal activity by using recombinant histatin-5 and its variants produced in Escherichia coli. The purified recombinant histatins were examined for their candidacidal activity and secondary structure. The m21 (with Lys-13 replaced by Thr [Lys-13-->Thr]) and m71 (Lys-13-->Glu) variants are significantly less effective than recombinant histatin-5 in killing Candida albicans, suggesting that Lys-13 is critical for candidacidal activity. The m68 (Lys-13-->Glu and Arg-22-->Gly) variant is significantly less potent than the recombinant histatin-5 as well as m71, indicating that Arg-22 is crucial for the cidal activity. The candidacidal activities of m1 (Arg-12-->Ile), m2 (Arg-12-->Ile and Lys-17-->Asp), m12 (Arg-12-->Lys and His-21-->Leu), and m70 (His-19-->Pro and His-21-->Arg) variants, however, are comparable to that of recombinant histatin-5, indicating that Arg-12, Lys-17, His-19, and His-21 are not functionally important. The conformational preferences of histatin-5 and variants were determined by circular dichroism. The results indicate that all proteins have a strong tendency to adopt alpha-helical conformation in trifluoroethanol. Previously, we have shown that the alpha-helical conformation is one of the important structural requirements for eliciting appreciable candidacidal activity. Collectively, the data suggest that in addition to the helical conformation, specific residues such as Lys-13 and Arg-22 in the sequence of histatin-5 are, indeed, important for candidacidal activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.