ArticleGut1996
Post-irradiation somatic mutation and clonal stabilisation time in the human colon.
Article in Gut, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 70 citations in OpenAlex.
- Accumulation of Oncogenic Mutations During Progression from Healthy Tissue to Cancer.Bulletin of mathematical biology · 2024Article
- Lineage tracing in human tissues.The Journal of pathology · 2022Review
- Mathematical model of colorectal cancer initiation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Evolutionary Stem Cell Poker and Cancer Risks: The Paradox of The Large And Small Intestines.Current pathobiology reports · 2018Article
- Fixation and Spread of Somatic Mutations in Adult Human Colonic Epithelium.Cell stem cell · 2018Article
- Heterogeneity of the level of activity of lgr5+ intestinal stem cells.International journal of molecular and cellular medicine · 2014Review
- Mutator/Hypermutable fetal/juvenile metakaryotic stem cells and human colorectal carcinogenesis.Frontiers in oncology · 2013Review
- Direct measurements of human colon crypt stem cell niche genetic fidelity: the role of chance in non-darwinian mutation selection.Frontiers in oncology · 2013Review
- ST6Gal-I protein expression is upregulated in human epithelial tumors and correlates with stem cell markers in normal tissues and colon cancer cell lines.Cancer research · 2013Article
- Large chromosome deletions, duplications, and gene conversion events accumulate with age in normal human colon crypts.Aging cell · 2013Article
- Article
- Review
- The stem cells of small intestinal crypts: where are they?Cell proliferation · 2009Article
- Stem cells in gastroenterology and hepatology.Nature reviews. Gastroenterology & hepatology · 2009Review
- Colon cancer stem cells.Journal of molecular medicine (Berlin, Germany) · 2009Review
- A new view of radiation-induced cancer: integrating short- and long-term processes. Part I: approach.Radiation and environmental biophysics · 2009Article
- Inferring human stem cell behaviour from epigenetic drift.The Journal of pathology · 2009Review
- The intestinal stem cell.Genes & development · 2008Review
- The stem cell population of the human colon crypt: analysis via methylation patterns.PLoS computational biology · 2007Article
- Stem cell chronicles: autobiographies within genomes.Stem cell reviews · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundColorectal crypts are clonal units in which somatic mutation of marker genes in stem cells leads to crypt restricted phenotypic conversion initially involving part of the crypt, later the whole crypt. Studies in mice show that the time taken for the great majority of mutated crypts to be completely converted, the clonal stabilisation time, is four weeks in the colon and 21 weeks in the ileum. Differences in the clonal stabilisation time between tissues and species are thought to reflect differences in stem cell organisation and crypt kinetics.
aimTo study the clonal stabilisation time in the human colorectum.
methodsStem cell mutation can lead to crypt restricted loss of O-acetylation of sialomucins in subjects heterozygous for O-acetyltransferase gene activity. mPAS histochemistry was used to visualise and quantify crypts partially or wholly involved by the mutant phenotype in 21 informative cases who had undergone colectomy up to 34 years after radiotherapy.
resultsRadiotherapy was followed by a considerable increase in the discordant crypt frequency that remained significantly increased for many years. The proportion of discordant crypts showing partial involvement was initially high but fell to normal levels about 12 months after irradiation.
conclusionsCrypts wholly involved by a mutant phenotype are stable and persistent while partially involved crypts are transient. The clonal stabilisation time is approximately one year in the human colon compared with four weeks in the mouse. The most likely reason for this is a difference in the number of stem cells in a crypt stem cell niche, although differences in stem cell cycle time and crypt fission may also contribute. These findings are of relevance to colorectal gene therapy and carcinogenesis in stem cell systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.