ArticleMolecular and cellular biology1996
Requirements for ectopic homologous recombination in mammalian somatic cells.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Nascent DNA synthesis during homologous recombination is synergistically promoted by the rad51 recombinase and DNA homology.Genetics · 2014Article
- Mph1 and Mus81-Mms4 prevent aberrant processing of mitotic recombination intermediates.Molecular cell · 2013Article
- A strand invasion 3' polymerization intermediate of mammalian homologous recombination.Genetics · 2010Article
- Article
- The biased distribution of Alus in human isochores might be driven by recombination.Journal of molecular evolution · 2005Article
- Conjugational genetic exchange in the hyperthermophilic archaeon Sulfolobus acidocaldarius: intragenic recombination with minimal dependence on marker separation.Journal of bacteriology · 2005Article
- Shuffling of genes within low-copy repeats on 22q11 (LCR22) by Alu-mediated recombination events during evolution.Genome research · 2003Article
- The effect of heterologous insertions on gene conversion in mitotically dividing cells in Drosophila melanogaster.Genetics · 2002Article
- Double-strand break-induced recombination between ectopic homologous sequences in somatic plant cells.Genetics · 1999Article
- Intrachromosomal recombination between well-separated, homologous sequences in mammalian cells.Genetics · 1999Article
- Factors affecting ectopic gene conversion in mice.Mammalian genome : official journal of the International Mammalian Genome Society · 1998Article
- Homology requirements for targeting heterologous sequences during P-induced gap repair in Drosophila melanogaster.Genetics · 1997Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ectopic recombination occurs between DNA sequences that are not in equivalent positions on homologous chromosomes and has beneficial as well as potentially deleterious consequences for the eukaryotic genome. In the present study, we have examined ectopic recombination in mammalian somatic (murine hybridoma) cells in which a deletion in the mu gene constant (Cmu) region of the endogenous chromosomal immunoglobulin mu gene is corrected by using as a donor an ectopic wild-type Cmu region. Ectopic recombination restores normal immunoglobulin M production in hybridomas. We show that (i) chromosomal mu gene deletions of 600 bp and 4 kb are corrected less efficiently than a deletion of only 2 bp, (ii) the minimum amount of homology required to mediate ectopic recombination is between 1.9 and 4.3 kb, (iii) the frequency of ectopic recombination does not depend on donor copy number, and (iv) the frequency of ectopic recombination in hybridoma lines in which the donor and recipient Cmu regions are physically connected to each other on the same chromosome can be as much as 4 orders of magnitude higher than it is for the same sequences located on homologous or nonhomologous chromosomes. The results are discussed in terms of a model for ectopic recombination in mammalian somatic cells in which the scanning mechanism that is used to locate a homologous partner operates preferentially in cis.
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