ArticleMolecular and cellular biology1996
Sequences within the 5' untranslated region regulate the levels of a kinetoplast DNA topoisomerase mRNA during the cell cycle.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Synthesis and characterization of I-BET151 derivatives for use in identifying protein targets in the African trypanosome.Current research in chemical biology · 2023Article
- Untranslated regions of mRNA and their role in regulation of gene expression in protozoan parasites.Journal of biosciences · 2017Review
- Article
- Bromodomain Proteins Contribute to Maintenance of Bloodstream Form Stage Identity in the African Trypanosome.PLoS biology · 2015Article
- Bloodstream form pre-adaptation to the tsetse fly in Trypanosoma brucei.Frontiers in cellular and infection microbiology · 2013Review
- The post-transcriptional trans-acting regulator, TbZFP3, co-ordinates transmission-stage enriched mRNAs in Trypanosoma brucei.Nucleic acids research · 2012Article
- Article
- Trans-acting proteins regulating mRNA maturation, stability and translation in trypanosomatids.Trends in parasitology · 2011Review
- Cell biology of the trypanosome genome.Microbiology and molecular biology reviews : MMBR · 2010Review
- Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.Nucleic acids research · 2010Article
- A mitochondrial DNA primase is essential for cell growth and kinetoplast DNA replication in Trypanosoma brucei.Molecular and cellular biology · 2010Article
- Trypanosoma brucei PUF9 regulates mRNAs for proteins involved in replicative processes over the cell cycle.PLoS pathogens · 2009Article
- Identification of new kinetoplast DNA replication proteins in trypanosomatids based on predicted S-phase expression and mitochondrial targeting.Eukaryotic cell · 2007Article
- Combining evidence, biomedical literature and statistical dependence: new insights for functional annotation of gene sets.BMC bioinformatics · 2006Article
- Cell cycle-dependent localization and properties of a second mitochondrial DNA ligase in Crithidia fasciculata.Eukaryotic cell · 2006Article
- Assigning functions to genes: identification of S-phase expressed genes in Leishmania major based on post-transcriptional control elements.Nucleic acids research · 2005Article
- Presence of a poly(A) binding protein and two proteins with cell cycle-dependent phosphorylation in Crithidia fasciculata mRNA cycling sequence binding protein II.Eukaryotic cell · 2004Article
- Sequence elements in both the intergenic space and the 3' untranslated region of the Crithidia fasciculata KAP3 gene are required for cell cycle regulation of KAP3 mRNA.Eukaryotic cell · 2003Article
- TbMP44 is essential for RNA editing and structural integrity of the editosome in Trypanosoma brucei.Eukaryotic cell · 2003Article
- Degradation of the unstable EP1 mRNA in Trypanosoma brucei involves initial destruction of the 3'-untranslated region.Nucleic acids research · 2001Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Gene expression in trypanosomatids appears to be regulated largely at the posttranscriptional level and involves maturation of mRNA precursors by trans splicing of a 39-nucleotide miniexon sequence to the 5' end of the mRNA and cleavage and polyadenylation at the 3' end of the mRNA. To initiate the identification of sequences involved in the periodic expression of DNA replication genes in trypanosomatids, we have mapped splice acceptor sites in the 5' flanking region of the TOP2 gene, which encodes the kinetoplast DNA topoisomerase, and have carried out deletion analysis of this region on a plasmid-encoded TOP2 gene. Block deletions within the 5' untranslated region (UTR) identified two regions (-608 to -388 and -387 to -186) responsible for periodic accumulation of the mRNA. Deletion of one or the other of these sequences had no effect on periodic expression of the mRNA, while deletion of both regions resulted in constitutive expression of the mRNA throughout the cell cycle. Subcloning of these sequences into the 5' UTR of a construct lacking both regions of the TOP2 5' UTR has shown that an octamer consensus sequence present in the 5' UTR of the TOP2, RPA1, and DHFR-TS mRNAs is required for normal cycling of the TOP2 mRNA. Mutation of the consensus octamer sequence in the TOP2 5' UTR in a plasmid construct containing only a single consensus octamer and that shows normal cycling of the plasmid-encoded TOP2 mRNA resulted in substantial reduction of the cycling of the mRNA level. These results imply a negative regulation of TOP2 mRNA during the cell cycle by a mechanism involving redundant elements containing one or more copies of a conserved octamer sequence within the 5' UTR of TOP2 mRNA.
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