ArticleProceedings of the National Academy of Sciences of the United States of America1996
A positive genetic selection for disrupting protein-protein interactions: identification of CREB mutations that prevent association with the coactivator CBP.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 101 citations in OpenAlex.
- PU.1-c-Jun interaction is crucial for PU.1 function in myeloid development.Communications biology · 2022Article
- Cytosolic pH regulates proliferation and tumour growth by promoting expression of cyclin D1.Nature metabolism · 2020Article
- Ectopic expression of aPKC-mediated phosphorylation in p300 modulates hippocampal neurogenesis, CREB binding and fear memory differently with age.Scientific reports · 2018Article
- Protein interaction perturbation profiling at amino-acid resolution.Nature methods · 2017Article
- The aPKC-CBP Pathway Regulates Adult Hippocampal Neurogenesis in an Age-Dependent Manner.Stem cell reports · 2016Article
- The cyclic AMP signaling pathway: Exploring targets for successful drug discovery (Review).Molecular medicine reports · 2016Review
- High-resolution network biology: connecting sequence with function.Nature reviews. Genetics · 2013Review
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Identification of residues in the N-terminal PAS domains important for dimerization of Arnt and AhR.Nucleic acids research · 2011Article
- 'Edgetic' perturbation of a C. elegans BCL2 ortholog.Nature methods · 2009Article
- Ca2+-dependent block of CREB-CBP transcription by repressor DREAM.The EMBO journal · 2002Article
- Dual roles of p300 in chromatin assembly and transcriptional activation in cooperation with nucleosome assembly protein 1 in vitro.Molecular and cellular biology · 2002Article
- MLL and CREB bind cooperatively to the nuclear coactivator CREB-binding protein.Molecular and cellular biology · 2001Article
- Dual requirement for rho and protein kinase C in direct activation of phospholipase D1 through G protein-coupled receptor signaling.Molecular biology of the cell · 2000Article
- Magnitude of the CREB-dependent transcriptional response is determined by the strength of the interaction between the kinase-inducible domain of CREB and the KIX domain of CREB-binding protein.Molecular and cellular biology · 2000Article
- Histone binding protein RbAp48 interacts with a complex of CREB binding protein and phosphorylated CREB.Molecular and cellular biology · 2000Article
- Recruitment of CREB binding protein is sufficient for CREB-mediated gene activation.Molecular and cellular biology · 2000Article
- Cubitus interruptus requires Drosophila CREB-binding protein to activate wingless expression in the Drosophila embryo.Molecular and cellular biology · 2000Article
- Yeast Two-Hybrid: State of the Art.Biological procedures online · 1999Article
- An exposed KID-like domain in human T-cell lymphotropic virus type 1 Tax is responsible for the recruitment of coactivators CBP/p300.Molecular and cellular biology · 1998Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The Escherichia coli tet-repressor (TetR) operator system was used to develop a variation of the yeast two-hybrid assay in which disruptions of protein-protein interactions can be identified by a positive selection. This assay, designated the "split-hybrid system," contains a two-component reporter. The first component contains LexA binding sites upstream of the TetR gene and the second contains TetR operator binding sites upstream of HIS3. Interaction of one protein fused to the LexA DNA binding domain with a second protein fused to the VP16 activation domain results in TetR expression. TetR subsequently binds to the tet operators, blocking the expression of HIS3 and preventing yeast growth in media lacking histidine. The utility of the split-hybrid system was analyzed by examining the phosphorylation-dependent interaction of CREB and its coactivator CREB binding protein (CBP). CREB and CBP associate through an interaction that depends upon CREB phosphorylation at Ser-133. Mutation of this phosphorylation site prevents yeast growth in the standard two-hybrid assay but allows growth in the split-hybrid strains. The split-hybrid system was used to identify other CREB mutations that disrupt its association with CBP. These mutations localized around the site of CREB phosphorylation, indicating that only a small portion of the CREB activation domain is required for CBP interaction. The yeast split-hybrid system should be useful in identifying mutations, proteins, peptides, and drugs that disrupt protein-protein interactions.
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