ArticleProceedings of the National Academy of Sciences of the United States of America1996
The structural H19 gene is required for transgene imprinting.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 93 citations in OpenAlex.
- Article
- Evidence for a functional role of Start, a long noncoding RNA, in mouse spermatocytes.PloS one · 2022Article
- Identification and computational analysis of gene regulatory elements.Cold Spring Harbor protocols · 2015Review
- The H19 long noncoding RNA gives rise to microRNAs miR-675-3p and miR-675-5p to promote skeletal muscle differentiation and regeneration.Genes & development · 2014Article
- The long reach of noncoding RNAs.Nature genetics · 2011Article
- BACs as tools for the study of genomic imprinting.Journal of biomedicine & biotechnology · 2011Review
- Long range interactions regulate Igf2 gene transcription during skeletal muscle differentiation.The Journal of biological chemistry · 2010Article
- Postnatal survival of mice with maternal duplication of distal chromosome 7 induced by a Igf2/H19 imprinting control region lacking insulator function.PLoS genetics · 2010Article
- Analysis of the H19ICR insulator.Molecular and cellular biology · 2007Article
- Article
- Specific differentially methylated domain sequences direct the maintenance of methylation at imprinted genes.Molecular and cellular biology · 2006Article
- Identification of a novel non-coding RNA, MIAT, that confers risk of myocardial infarction.Journal of human genetics · 2006Article
- Expression of a modified H19 RNA does not cause embryonic lethality in mice.Mammalian genome : official journal of the International Mammalian Genome Society · 2006Article
- Tandem repeat hypothesis in imprinting: deletion of a conserved direct repeat element upstream of H19 has no effect on imprinting in the Igf2-H19 region.Molecular and cellular biology · 2004Article
- The H19 differentially methylated region marks the parental origin of a heterologous locus without gametic DNA methylation.Molecular and cellular biology · 2004Article
- IGF-II transcription in skeletal myogenesis is controlled by mTOR and nutrients.The Journal of cell biology · 2003Article
- Analysis of sequence upstream of the endogenous H19 gene reveals elements both essential and dispensable for imprinting.Molecular and cellular biology · 2002Article
- Regulatory mechanisms at the mouse Igf2/H19 locus.Molecular and cellular biology · 2001Article
- Investigation of elements sufficient to imprint the mouse Air promoter.Molecular and cellular biology · 2001Article
- Size matters: use of YACs, BACs and PACs in transgenic animals.Transgenic research · 2001Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The product of the H19 gene is an untranslated RNA that is expressed exclusively from the maternal chromosome during mammalian development. The H19 gene and its 5'-flanking sequence are required for the genomic imprinting of two paternally expressed genes, Ins-2 (encodes insulin-2) and Igf-2 (encodes insulin-like growth factor-2), that lie 90 and 115 kb 5' to the H19 gene, respectively. In this report, the role of the H19 gene in its own imprinting is investigated by introducing a Mus spretus H19 gene into heterologous locations in the mouse genome. Multiple copies of the transgene were sufficient for its paternal silencing and DNA methylation. Replacing the H19 structural gene with a luciferase reporter gene resulted in loss of imprinting of the transgene. That is, high expression and low levels of DNA methylation were observed upon both paternal and maternal inheritance. The removal of 701 bp at the 5' end of the structural gene resulted in a similar loss of paternal-specific DNA methylation, arguing that those sequences are required for both the establishment and maintenance of the sperm-specific gametic mark. The M. spretus H19 transgene could not rescue the loss of Igf-2 imprinting in trans in H19 deletion mice, implying a cis requirement for the H19 gene. In contrast to a previous report in which overexpression of a marked H19 gene was a prenatal lethal, expression of the M. spretus transgene had no deleterious effect, leading to the conclusion that the 20-base insertion in the marked gene created a neomorphic mutation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.