ArticleProceedings of the National Academy of Sciences of the United States of America1996
Sucrose biosynthesis in a prokaryotic organism: Presence of two sucrose-phosphate synthases in Anabaena with remarkable differences compared with the plant enzymes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 83 citations in OpenAlex.
- Architecture, Function, Regulation, and Evolution of α-Glucans Metabolic Enzymes in Prokaryotes.Chemical reviews · 2024Review
- Cyanobacteria as cell factories for the photosynthetic production of sucrose.Frontiers in microbiology · 2023Review
- Altered Carbon Partitioning Enhances COBiodesign research · 2022Article
- Mutation of UDP-glucose binding motif residues lead to increased affinity for ADP-glucose in sugarcane sucrose phosphate synthase.Molecular biology reports · 2021Article
- Salt-Regulated Accumulation of the Compatible Solutes Sucrose and Glucosylglycerol in Cyanobacteria and Its Biotechnological Potential.Frontiers in microbiology · 2019Review
- Sucrose in cyanobacteria: from a salt-response molecule to play a key role in nitrogen fixation.Life (Basel, Switzerland) · 2015Review
- Salt acclimation of cyanobacteria and their application in biotechnology.Life (Basel, Switzerland) · 2014Review
- Sucrose synthesis in the nitrogen-fixing Cyanobacterium Anabaena sp. strain PCC 7120 is controlled by the two-component response regulator OrrA.Applied and environmental microbiology · 2014Article
- Biodesalination: a case study for applications of photosynthetic bacteria in water treatment.Plant physiology · 2014Review
- Cell-specific gene expression in Anabaena variabilis grown phototrophically, mixotrophically, and heterotrophically.BMC genomics · 2013Article
- Article
- Sucrose synthase in unicellular cyanobacteria and its relationship with salt and hypoxic stress.Planta · 2012Article
- Inactivation of a heterocyst-specific invertase indicates a principal role of sucrose catabolism in heterocysts of Anabaena sp.Journal of bacteriology · 2010Article
- Article
- Trehalose-producing enzymes MTSase and MTHase in Anabaena 7120 under NaCl stress.Current microbiology · 2008Article
- The structure of sucrose phosphate synthase from Halothermothrix orenii reveals its mechanism of action and binding mode.The Plant cell · 2008Article
- A metabolic pathway leading to mannosylfructose biosynthesis in Agrobacterium tumefaciens uncovers a family of mannosyltransferases.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Carbon cycling in Anabaena sp. PCC 7120. Sucrose synthesis in the heterocysts and possible role in nitrogen fixation.Plant physiology · 2007Article
- Biosynthetic pathways of inositol and glycerol phosphodiesters used by the hyperthermophile Archaeoglobus fulgidus in stress adaptation.Journal of bacteriology · 2006Article
- Cyanobacterial alkaline/neutral invertases. Origin of sucrose hydrolysis in the plant cytosol?Planta · 2003Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Biosynthesis of sucrose-6-P catalyzed by sucrose-phosphate synthase (SPS), and the presence of sucrose-phosphate phosphatase (SPP) leading to the formation of sucrose, have both been ascertained in a prokaryotic organism: Anabaena 7119, a filamentous heterocystic cyanobacterium. Two SPS activities (SPS-I and SPS-II) were isolated by ion-exchange chromatography and partially purified. Four remarkable differences between SPSs from Anabaena and those from higher plants were shown: substrate specificity, effect of divalent cations, native molecular mass, and oligomeric composition. Both SPS-I and SPS-II accept Fru-6-P (K(m) for SPS-I = 0.8 +/- 0.1 mM; K(m) for SPS-II = 0.7 +/- 0.1 mM) and UDP-Glc as substrates (K(m) for SPS-I = 1.3 +/- 0.4 mM; K(m) for SPS-II = 4.6 +/- 0.4 mM), but unlike higher plant enzymes, they are not specific for UDP-Glc. GDP-Glc and TDP-Glc are also SPS-I substrates (K(m) for GDP-Glc = 1.2 +/- 0.2 mM and K(m) for TDP-Glc = 4.0 +/- 0.4 mM), and ADP-Glc is used by SPS-II (K(m) for ADP-Glc = 5.7 +/- 0.7 mM). SPS-I has an absolute dependence toward divalent metal ions (Mg2+ or Mn2+) for catalytic activity, not found in plants. A strikingly smaller native molecular mass (between 45 and 47 kDa) was determined by gel filtration for both SPSs, which, when submitted to SDS/PAGE, showed a monomeric composition. Cyanobacteria are, as far as the authors know, the most primitive organisms that are able to biosynthesize sucrose as higher plants do.
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