ArticleThe Journal of clinical investigation1996
Muscle differentiation during repair of myocardial necrosis in rats via gene transfer with MyoD.
Article in The Journal of clinical investigation, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 144 citations in OpenAlex.
- Cardiac commitment driven by MyoD expression in pericardial stem cells.Frontiers in cell and developmental biology · 2024Article
- Genome Editing and Cardiac Regeneration.Advances in experimental medicine and biology · 2023Article
- Review
- Lineage tracing of direct astrocyte-to-neuron conversion in the mouse cortex.Neural regeneration research · 2021Article
- Systematic Approach to Developing Splice Modulating Antisense Oligonucleotides.International journal of molecular sciences · 2019Article
- Cardiac progenitor reprogramming for heart regeneration.Cell regeneration (London, England) · 2018Review
- Mechanisms of Cardiac Repair and Regeneration.Circulation research · 2018Review
- Optimizing stem cells for cardiac repair: Current status and new frontiers in regenerative cardiology.World journal of stem cells · 2017Review
- Engineering Cell Fate for Tissue Regeneration by In Vivo Transdifferentiation.Stem cell reviews and reports · 2016Review
- Small mammalian animal models of heart disease.American journal of cardiovascular disease · 2016Review
- Direct Cardiac Reprogramming: Advances in Cardiac Regeneration.BioMed research international · 2015Review
- Heart regeneration, stem cells, and cytokines.Regenerative medicine research · 2014Review
- Pitx2c is reactivated in the failing myocardium and stimulates myf5 expression in cultured cardiomyocytes.PloS one · 2014Article
- Is it possible to transform cardiac scar tissue into beating heart muscle in humans?Regenerative medicine · 2012Review
- Article
- Article
- Heart regeneration.Nature · 2011Review
- Reprogramming fibroblasts into cardiomyocytes.The New England journal of medicine · 2011Article
- Stem cell paracrine actions and tissue regeneration.Regenerative medicine · 2010Review
- Angiomyogenesis for myocardial repair.Antioxidants & redox signaling · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Myocardial infarcts heal by scar formation because there are no stem cells in myocardium, and because adult myocytes cannot divide and repopulate the wound. We sought to redirect the heart to form skeletal muscle instead of scar by transferring the myogenic determination gene, MyoD, into cardiac granulation (wound repair) tissue. A replication-defective adenovirus was constructed containing MyoD under transcriptional control of the Rous sarcoma virus long terminal repeat. The virus converted cultured cardiac fibroblasts to skeletal muscle, indicated by expression of myogenin and skeletal myosin heavy chains (MHCs). To determine if MyoD could induce muscle differentiation in vivo, we injected 2 x 10(9) or 10(10) pfu of either the MyoD or a control beta-galactosidase adenovirus into healing rat hearts, injured 1 wk previously by freeze-thaw. After receiving the lower viral dose, cardiac granulation tissue expressed MyoD mRNA and protein, but did not express myogenin or skeletal MHC. When the higher dose of virus was administered, double immunostaining showed that cells in reparative tissue expressed both myogenin and embryonic skeletal MHC. No muscle differentiation occurred after beta-galactosidase transfection. Thus, MyoD gene transfer can induce skeletal muscle differentiation in healing heart lesions. Modifications of this strategy might eventually provide new contractile tissue to repair myocardial infarcts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.