ArticleJournal of clinical microbiology1996
Differentiation of bacterial 16S rRNA genes and intergenic regions and Mycobacterium tuberculosis katG genes by structure-specific endonuclease cleavage.
Article in Journal of clinical microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 72 citations in OpenAlex.
- DNA polymerase I: structure, activity, and function in bacterial DNA replication and repair.Critical reviews in biochemistry and molecular biology · 2026Review
- DNA terminal structure-mediated enzymatic reaction for ultra-sensitive discrimination of single nucleotide variations in circulating cell-free DNA.Nucleic acids research · 2018Article
- The bacterial community of the lithistid sponge Discodermia spp. as determined by cultivation and culture-independent methods.Marine biotechnology (New York, N.Y.) · 2012Article
- Molecular diagnostics of clinically important staphylococci.Folia microbiologica · 2004Review
- Detection of isoniazid-resistant Mycobacterium tuberculosis strains by a multiplex allele-specific PCR assay targeting katG codon 315 variation.Journal of clinical microbiology · 2002Article
- High prevalence of KatG Ser315Thr substitution among isoniazid-resistant Mycobacterium tuberculosis clinical isolates from northwestern Russia, 1996 to 2001.Antimicrobial agents and chemotherapy · 2002Article
- Detection of germline BRCA1 mutations by Multiple-Dye Cleavase Fragment Length Polymorphism (MD-CFLP) method.British journal of cancer · 2001Article
- Secondary structure prediction and structure-specific sequence analysis of single-stranded DNA.Nucleic acids research · 2001Article
- Identification of drug-resistantMycobacterium tuberculosis by single strand conformation polymorphism and cleavase fragment length polymorphism.Indian journal of clinical biochemistry : IJCB · 2000Article
- Cleavase fragment length polymorphism analysis of Neisseria meningitidis basic metabolic genes.Journal of clinical microbiology · 1999Article
- Principles and applications of methods for DNA-based typing of microbial organisms.Journal of clinical microbiology · 1999Review
- Genetic methods for assessing antimicrobial resistance.Antimicrobial agents and chemotherapy · 1999Review
- Algorithmic approach to high-throughput molecular screening for alpha interferon-resistant genotypes in hepatitis C patients.Journal of clinical microbiology · 1998Article
- Determination of hepatitis C virus genotypes in the United States by cleavase fragment length polymorphism analysis.Journal of clinical microbiology · 1997Article
- Diversity of the bacterial communities associated with the azooxanthellate deep water octocorals Leptogorgia minimata, Iciligorgia schrammi, and Swiftia exertia.Marine biotechnology (New York, N.Y.)Article
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Authors and funding
11 authors at 2 institutions in 1 country.
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Abstract
We describe here a new approach for analyzing nucleic acid sequences using a structure-specific endonuclease, Cleavase I. We have applied this technique to the detection and localization of mutations associated with isoniazid resistance in Mycobacterium tuberculosis and for differentiating bacterial genera, species and strains. The technique described here is based on the observation that single strands of DNAs can assume defined conformations, which can be detected and cleaved by structure-specific endonucleases such as Cleavase I. The patterns of fragments produced are characteristic of the sequences responsible for the structure, so that each DNA has its own structural fingerprint. Amplicons, containing either a single 5'-fluorescein or 5'-tetramethyl rhodamine label were generated from a 620-bp segment of the katG gene of isoniazid-resistant and -sensitive M. tuberculosis, the 5' 350 bp of the 16S rRNA genes of Escherichia coli O157:H7, Salmonella typhimurium, Salmonella enteritidis, Salmonella arizonae, Shigella sonnei, Shigella dysenteriae, Campylobacter jejuni, staphylococcus, hominis, Staphylococcus warneri, and Staphylococcus aureus and an approximately 550-bp DNA segment comprising the intergenic region between the 16S and 23S rRNA genes of Salmonella typhimurium, Salmonella enteritidis, Salmonella arizonae, Shigella sonnei, and Shigella dysenteriae serotypes 1, 2, and 8. Changes in the structural fingerprints of DNA fragments derived from the katG genes of isoniazid-resistant M. tuberculosis isolates were clearly identified and could be mapped to the site of the actual mutation relative to the labeled end. Bland patterns which clearly differentiated bacteria to the level of genus and, in some cases, species were generated from the 16S genes. Cleavase I analysis of the intergenic regions of Salmonella and Shigella species differentiated genus, species, and serotypes. Structural fingerprinting by digestion with Cleavase I is a rapid, simple, and sensitive method for analyzing nucleic acid sequences and may find wide utility in microbial analysis.
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